Immunohistochemical expression of FHIT gene product in inflammatory bowel disease: significance and correlation with clinicopathological data

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dc.contributor.authorSkopelitou, A. S.en
dc.contributor.authorKatsanos, K. H.en
dc.contributor.authorMichail, M.en
dc.contributor.authorMitselou, A.en
dc.contributor.authorTsianos, E. V.en
dc.date.accessioned2015-11-24T19:11:58Z
dc.date.available2015-11-24T19:11:58Z
dc.identifier.issn0954-691X-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20990
dc.rightsDefault Licence-
dc.subject*Acid Anhydride Hydrolasesen
dc.subjectAdenocarcinoma/etiology/metabolismen
dc.subjectAdolescenten
dc.subjectAdulten
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectColitis, Ulcerative/complications/metabolism/pathologyen
dc.subjectColon/metabolismen
dc.subjectColorectal Neoplasms/etiology/metabolismen
dc.subjectCrohn Disease/complications/metabolism/pathologyen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectImmunoenzyme Techniquesen
dc.subjectInflammatory Bowel Diseases/*metabolism/pathologyen
dc.subjectIntestinal Mucosa/metabolismen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectNeoplasm Proteins/*metabolismen
dc.titleImmunohistochemical expression of FHIT gene product in inflammatory bowel disease: significance and correlation with clinicopathological dataen
heal.abstractOBJECTIVE: To investigate the expression of Fhit protein in 53 patients with ulcerative colitis and Crohn's disease, as well as in 13 ulcerative-colitis-associated adenocarcinomas, and its eventual relationship with clinicopathological data and response to therapy. MATERIALS AND METHODS: We performed immunohistochemistry in archival material of formalin-fixed, paraffin-embedded tissues using the anti-Fhit antibody and the streptavidin-biotin peroxidase method. RESULTS: In 35/38 cases of ulcerative colitis, Fhit protein (pFhit) was absent or reduced. In the remaining three cases, it was expressed normally. In 12/15 cases of Crohn's disease, pFhit was absent or reduced, as it was in 4/13 cases of ulcerative-colitis-associated adenocarcinoma. Statistically significant differences of pFhit expression were observed between the active phase and the chronic healed phase of ulcerative colitis, and between the active phase and the normal colon mucosa. Also, statistically significant differences of pFhit expression were observed between (1) the resolving phase of ulcerative colitis and normal colon mucosa, (2) the chronic healed phase and normal colon mucosa, and (3) Crohn's disease and normal colon mucosa. Interestingly, a statistically very significant difference in pFhit expression was noticed between ulcerative colitis and ulcerative-colitis-associated adenocarcinoma. CONCLUSIONS: Our results show that immunohistochemical expression of pFhit is completely absent or very reduced in idiopathic bowel disease (IBD) as well as in several ulcerative-colitis-associated adenocarcinomas in north-western Greece. These findings suggest that the FHIT gene might be involved in IBD and in a subgroup of ulcerative-colitis-associated carcinogenesis, although larger series should be tested.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1097/01.meg.0000059122.68845.0b-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12840679-
heal.journalNameEur J Gastroenterol Hepatolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2003-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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