Analysis of tamoxifen and 4-hydroxytamoxifen levels in immature rat uterine cytoplasm and KCl-nuclear extracts by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM)
| dc.contributor.author | Murphy, C. | en |
| dc.contributor.author | Fotsis, T. | en |
| dc.contributor.author | Adlercreutz, H. | en |
| dc.contributor.author | Martin, F. | en |
| dc.date.accessioned | 2015-11-24T19:36:21Z | |
| dc.date.available | 2015-11-24T19:36:21Z | |
| dc.identifier.issn | 0022-4731 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23859 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Cytosol/metabolism | en |
| dc.subject | Estrogen Antagonists/*pharmacokinetics | en |
| dc.subject | Female | en |
| dc.subject | Gas Chromatography-Mass Spectrometry/methods | en |
| dc.subject | Rats | en |
| dc.subject | Rats, Inbred Strains | en |
| dc.subject | Tamoxifen/*analogs & derivatives/*pharmacokinetics | en |
| dc.subject | Tritium | en |
| dc.subject | Uterus/*metabolism | en |
| dc.title | Analysis of tamoxifen and 4-hydroxytamoxifen levels in immature rat uterine cytoplasm and KCl-nuclear extracts by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM) | en |
| heal.abstract | A GC-MS (SIM) method has been developed which allows the measurement of tamoxifen and its metabolites in uterine cytosol and 0.5 M KCl-extracts of uterine nuclei from groups of immature rats. The method was shown to be specific, precise and accurate. Using this procedure tamoxifen and 4-hydroxytamoxifen were tentatively identified and measured in uterine extracts after tamoxifen administration. When tamoxifen and 4-hydroxytamoxifen levels in uterine cytosol and 0.5 M KCl nuclear extracts were compared a relative enrichment in the nuclear fraction of 4-hydroxytamoxifen (relative to tamoxifen) was consistently seen. These observations are supportive of a role for 4-hydroxytamoxifen in mediating the antiestrogenic actions of tamoxifen in the immature rat uterus. In some of the uterine cytosolic fractions desmethyltamoxifen and metabolite Y could also be detected. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/3657151 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/0022473187910211/1-s2.0-0022473187910211-main.pdf?_tid=6dc82eeb2e8dfcf90859feb6812d9ddf&acdnat=1332753026_1708661ecc2e98812e88c1ee372650e7 | - |
| heal.journalName | J Steroid Biochem | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1987 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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