Impaired phospholipases A(2) production by stimulated macrophages from patients with acute respiratory distress syndrome
Φόρτωση...
Ημερομηνία
Συγγραφείς
Hatzidaki, E.
Nakos, G.
Galiatsou, E.
Lekka, M. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Biochimica Et Biophysica Acta-Molecular Basis of Disease
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The aim of this study was to investigate whether early phase of acute respiratory distress syndrome (ARDS) is associated with changes in immune response, either systemic or localized to the lung. ARDS and control mechanically ventilated patients, as well as healthy volunteers were studied. Alveolar macrophages (AM phi) and blood monocytes (BM) were treated ex vivo with lipopolysaccharide (LPS), interferon-gamma (IFN-gamma), and surfactant. Phospholipase A(2) (PLA(2)) activity and TLR4 expression were evaluated as markers of cell response. AM phi from ARDS patients did not respond upon treatment with either LPS or IFN-gamma by inducing PLA(2) production On the contrary, upon stimulation, In control patients the intracellular PLA(2). (mainly cPLA(2)) levels were increased, but secretion of PLA(2) (mainly sPLA(2)-IIA) was observed only after treatment with LPS. Surfactant suppressed PLA(2) production in cells from both groups of patients Increased relative changes of total PLA(2) activity and an upregulation of TLR4 expression upon stimulation was observed in BM from primary ARDS, control patients and healthy volunteers. In BM from secondary ARDS patients, however, no PLA(2) induction was observed, with a concomitant down-regulation of TLR4 expression. Cytosolic PLA(2). its activated form, p-cPLA(2). and sPLA(2)-IIA were the predominant PLA(2) types within the cells, while extracellularly only sPLA(2)-IIA was identified. These results support the concept of down-regulated Innate immunity in early ARDS that is compartmentalized in primary and systemic in secondary ARDS. PLA(2) isoforms could serve as markers of the immunity status in ARDS. Finally, our data highlight the role of surfactant in controlling inflammation (C) 2010 Elsevier B V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
ards, phospholipase a(2), alveolar macrophage, lung surfactant, immune tolerance, surfactant protein-a, multiple organ failure, interferon-gamma, lung inflammation, epithelial-cells, ifn-gamma, group-iia, kappa-b, group-x, group-v
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000282616400007
http://ac.els-cdn.com/S0925443910001183/1-s2.0-S0925443910001183-main.pdf?_tid=aac6a5d7196484d9693d2cf9553d009d&acdnat=1333033649_890fcaf18a93d84bd40c0638bb1fc9a6
http://ac.els-cdn.com/S0925443910001183/1-s2.0-S0925443910001183-main.pdf?_tid=aac6a5d7196484d9693d2cf9553d009d&acdnat=1333033649_890fcaf18a93d84bd40c0638bb1fc9a6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας