NF-kappa B modulates TNF-alpha production by alveolar macrophages in asymptomatic HIV-seropositive individuals

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dc.contributor.authorMathys, J. M.en
dc.contributor.authorMelanson, S. M.en
dc.contributor.authorSchiffer-Alberts, D. J.en
dc.contributor.authorIoannidis, J. P.en
dc.contributor.authorKoziel, H.en
dc.contributor.authorSkolnik, P. R.en
dc.date.accessioned2015-11-24T19:02:01Z
dc.date.available2015-11-24T19:02:01Z
dc.identifier.issn0022-1767-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19751
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectCell Adhesion/genetics/immunologyen
dc.subjectDNA-Binding Proteins/metabolismen
dc.subjectFemaleen
dc.subjectHIV Seropositivity/*immunologyen
dc.subjectHumansen
dc.subject*I-kappa B Proteinsen
dc.subjectMacrophages, Alveolar/*immunology/*metabolismen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectNF-kappa B/genetics/metabolism/*physiologyen
dc.subjectPolymerase Chain Reactionen
dc.subjectPrimed In Situ Labelingen
dc.subjectProspective Studiesen
dc.subjectRNA, Messenger/antagonists & inhibitors/biosynthesisen
dc.subjectSerine Proteinase Inhibitors/pharmacologyen
dc.subjectSignal Transduction/drug effects/immunologyen
dc.subjectTosylphenylalanyl Chloromethyl Ketone/pharmacologyen
dc.subjectTumor Necrosis Factor-alpha/antagonists & inhibitors/*biosynthesis/geneticsen
dc.subjectType C Phospholipases/antagonists & inhibitorsen
dc.subjectTyrosine/analogs & derivatives/pharmacologyen
dc.titleNF-kappa B modulates TNF-alpha production by alveolar macrophages in asymptomatic HIV-seropositive individualsen
heal.abstractLocal TNF-alpha production in different organs may affect HIV replication and pathogenesis. Alveolar macrophages (AMs) obtained by bronchoalveolar lavage from asymptomatic HIV-seropositive and HIV-seronegative individuals did not spontaneously release TNF-alpha, but LPS stimulation of these cells significantly increased TNF-alpha production. We tested whether NF-kappa B affects TNF-alpha production by AMs using N-tosyl-<cmd SC>l<cmd /SC> -phenylalanine chloromethylketone (TPCK) or N-benzoyl-<cmd SC>l<cmd /SC> -tyrosine ethyl ester (BTEE), which inhibit the degradation of I kappa B, or tricyclodecan-9-yl-xanthogenate-potassium (D609), which inhibits phospholipase C. Alveolar macrophages were exposed to LPS alone and with the chemical protease inhibitors TPCK, BTEE, and D609. NF-kappa B DNA binding induced by LPS treatment of AMs was inhibited by TPCK, BTEE, and D609. These agents also inhibited TNF-alpha mRNA and TNF-alpha protein production. After 24 h, the levels of TNF-alpha mRNA reached equilibrium, as assessed by RT-PCR. The levels of NF-kappa B mRNA remained constant under all conditions. The levels of I kappa B-alpha mRNA were similar after 30, 60, and 180 min, but the I kappa B-beta mRNA concentration was initially low and increased over time under all conditions. I kappa B-alpha and I kappa B-beta protein production was not affected by the chemical protease inhibitors. Our data show that TNF-alpha production by LPS-stimulated AMs from asymptomatic HIV-seropositive and -seronegative individuals is regulated via the phospholipase C pathway and by NF-kappa B DNA binding activity without obvious changes in I kappa B-alpha or I kappa B-beta protein concentrations.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10640779-
heal.identifier.secondaryhttp://www.jimmunol.org/content/164/3/1588.full.pdf-
heal.journalNameJ Immunolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2000-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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