Neuroligand-triggered calcium signalling in cultured human glioma cells
| dc.contributor.author | Weydt, P. | en |
| dc.contributor.author | Moller, T. | en |
| dc.contributor.author | Labrakakis, C. | en |
| dc.contributor.author | Patt, S. | en |
| dc.contributor.author | Kettenmann, H. | en |
| dc.date.accessioned | 2015-11-24T16:31:50Z | |
| dc.date.available | 2015-11-24T16:31:50Z | |
| dc.identifier.issn | 0304-3940 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7488 | |
| dc.rights | Default Licence | - |
| dc.subject | Adenosine Triphosphate/pharmacology | en |
| dc.subject | Adrenergic alpha-Agonists/pharmacology | en |
| dc.subject | Angiotensin II/pharmacology | en |
| dc.subject | Bradykinin/pharmacology | en |
| dc.subject | Calcium/*metabolism | en |
| dc.subject | Fluorescent Dyes | en |
| dc.subject | Glioblastoma | en |
| dc.subject | Glucose/pharmacology | en |
| dc.subject | Histamine/pharmacology | en |
| dc.subject | Humans | en |
| dc.subject | Neurotransmitte | en |
| dc.title | Neuroligand-triggered calcium signalling in cultured human glioma cells | en |
| heal.abstract | Cells from primary cultures of four glioblastomas (GB), three low-grade astrocytomas (A), and four low-grade oligodendrogliomas (O) were tested for the presence of neuroligand receptors linked to Ca2+ signalling by calcium imaging. Cells of days 3 to 21 in culture were incubated with 5 microM fluo-3-acetomethylester in a bath solution and stimulated with 0.1 mM ATP, 0.01 mM angiotensin II, bradykinin, histamine, norepinephrine, serotonin, and substance P for 15 s, with 0.01 mM glutamate and 50 mM K+ for 30 s. Changes in the Ca2+ concentration were measured with a confocal laser scanning microscope. In all glioma subtypes, the majority of cells showed Ca2+ responses after application of histamine (60% of cells tested in GB, 67% in A, 86% in O), bradykinin (66% in GB, 29% in A, 55% in O) and ATP (48% in GB, 70% in A, 47% in O). The other stimuli induced Ca2+ transients in a smaller proportion (between 33% and 2%) of the cells. Our study demonstrates that histamine, bradykinin and ATP are potent inducers of [Ca2+]i signals in gliomas. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/9209106 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0304394097003662/1-s2.0-S0304394097003662-main.pdf?_tid=2d1dc664-c388-11e2-8905-00000aacb35d&acdnat=1369300221_6bd3724fd5f78ee57a021b4d53c20814 | - |
| heal.journalName | Neurosci Lett | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1997 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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