Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1)
| dc.contributor.author | Marangos, P. | en |
| dc.contributor.author | Verschuren, E. W. | en |
| dc.contributor.author | Chen, R. | en |
| dc.contributor.author | Jackson, P. K. | en |
| dc.contributor.author | Carroll, J. | en |
| dc.date.accessioned | 2015-11-24T16:33:51Z | |
| dc.date.available | 2015-11-24T16:33:51Z | |
| dc.identifier.issn | 0021-9525 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7723 | |
| dc.rights | Default Licence | - |
| dc.subject | 3T3 Cells | en |
| dc.subject | Animals | en |
| dc.subject | Cell Cycle Proteins/*metabolism | en |
| dc.subject | Cyclin B/metabolism | en |
| dc.subject | Embryo, Mammalian/cytology/metabolism | en |
| dc.subject | F-Box Proteins/*metabolism | en |
| dc.subject | Female | en |
| dc.subject | Gene Expression Regulation | en |
| dc.subject | Humans | en |
| dc.subject | Meiosis | en |
| dc.subject | *Meiotic Prophase I | en |
| dc.subject | *Metaphase | en |
| dc.subject | Mice | en |
| dc.subject | Mitotic Spindle Appa | en |
| dc.title | Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1) | en |
| heal.abstract | Mammalian oocytes are arrested in prophase of the first meiotic division. Progression into the first meiotic division is driven by an increase in the activity of maturation-promoting factor (MPF). In mouse oocytes, we find that early mitotic inhibitor 1 (Emi1), an inhibitor of the anaphase-promoting complex (APC) that is responsible for cyclin B destruction and inactivation of MPF, is present at prophase I and undergoes Skp1-Cul1-F-box/betaTrCP-mediated destruction immediately after germinal vesicle breakdown (GVBD). Exogenous Emi1 or the inhibition of Emi1 destruction in prophase-arrested oocytes leads to a stabilization of cyclin B1-GFP that is sufficient to trigger GVBD. In contrast, the depletion of Emi1 using morpholino oligonucleotides increases cyclin B1-GFP destruction, resulting in an attenuation of MPF activation and a delay of entry into the first meiotic division. Finally, we show that Emi1-dependent effects on meiosis I require the presence of Cdh1. These observations reveal a novel mechanism for the control of entry into the first meiotic division: an Emi1-dependent inhibition of APC(Cdh1). | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1083/jcb.200607070 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/17190794 | - |
| heal.journalName | J Cell Biol | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2007 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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