Lipoprotein-associated phospholipase A(2) activity is a marker of small, dense LDL particles in human plasma

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dc.contributor.authorGazi, I.en
dc.contributor.authorLourida, E. S.en
dc.contributor.authorFilippatos, T.en
dc.contributor.authorTsimihodimos, V.en
dc.contributor.authorElisaf, M.en
dc.contributor.authorTselepis, A. D.en
dc.date.accessioned2015-11-24T16:41:10Z
dc.date.available2015-11-24T16:41:10Z
dc.identifier.issn0009-9147-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8376
dc.rightsDefault Licence-
dc.subjectactivating-factor-acetylhydrolaseen
dc.subjectcoronary-heart-diseaseen
dc.subjectc-reactive proteinen
dc.subjectmiddle-aged menen
dc.subjectartery-diseaseen
dc.subjectdegrading acetylhydrolaseen
dc.subjectoxidative susceptibilityen
dc.subjectrisk-factorsen
dc.subjectfollow-upen
dc.subjectatherosclerosisen
dc.titleLipoprotein-associated phospholipase A(2) activity is a marker of small, dense LDL particles in human plasmaen
heal.abstractBackground: Recent, clinical studies showed that lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) is a predictor for incident atherosclerotic disease. We have previously shown that among the LDL subfractions, Lp-PLA(2) activity is preferentially associated with the atherogenic small, dense (sdLDL) particles in vitro. We investigated whether Lp-PLA(2) could be a marker of sdLDL in human plasma. Methods: One hundred and seventy-six individuals participated in the study. LDL subclass analysis was performed by polyacrylamide gel electrophoresis. LpPLA(2) activity and mass were determined in total plasma and in apolipoprotein B-depleted plasma (HDL-LpPLA(2)). Non-HDL-Lp-PLA(2) activity and mass were calculated by subtracting the HDL-Lp-PLA(2) from total plasma Lp-PLA(2). Results: On the basis of the LDL subclass analysis, participants were categorized into phenotype A and non-A (total cholesterol mass of the sdLDL subfractions <= 0.155 and >0.155 mmol/L, respectively). Unlike total plasma Lp-PLA(2) mass, total plasma Lp-PLA(2) activity and non-HDL-Lp-PLA(2) activity and mass were significantly higher in persons with phenotype non-A compared with persons with phenotype A, whereas HDLLp-PLA, activity and mass were lower in persons with phenotype non-A compared with phenotype A. Total plasma activity and non-HDL-Lp-PLA(2) activity and mass, but not Lp-PLA(2) mass, were correlated with sdLDL-cholesterol mass, proportion, and mean LDL particle size. In multiple regression analysis, total plasma and non-HDL-Lp-PLA(2) activities were the second best predictors of the presence of sdLDL particles in human plasma after serum triglyceride concentrations. At serum triglyceride concentrations >1.356 mmol/L, total plasma and non-HDL-Lp-PLA(2) activity added significantly to the prediction of the presence of sdLDL in plasma. Conclusions: Lp-PLA2 activity, but not the enzyme mass, is a marker of sdLDL in human plasma. (c) 2005 American Association for Clinical Chemistry.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1373/clinchem.2005.058404-
heal.identifier.secondary<Go to ISI>://000233533300009-
heal.identifier.secondaryhttp://www.clinchem.org/content/51/12/2264.full.pdf-
heal.journalNameClin Chemen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2005-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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