Demonstration of nitric oxide synthase (NOS) in marmosets by NADPH diaphorase (NADPH-d) histochemistry and NOS immunoreactivity
Φόρτωση...
Ημερομηνία
Συγγραφείς
Grozdanovic, Z.
Nakos, G.
Christova, T.
Nikolova, Z.
Mayer, B.
Gossrau, R.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Acta Histochem
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Since species interdiversity often prevents the extrapolation of laboratory rodent data to man and similar problems may exist for nitric oxide synthase (NOS), NADPH-d activity and immunohistochemistry of NOS were investigated in the New World monkey Callithrix jacchus (marmoset), which has been shown to be close to the human situation in many respects. Using the NADPHd reaction with beta-NADPH and nitroblue tetrazolium (NBT) on acetone-chloroform pretreated cryosections, NBT formazan was found in many neural and non-neural (e.g. diverse epithelia, striated muscle fibers, vascular endothelium) cells in numerous tissues and organs. Prefixation with formaldehyde lowered the number of NADPH-d active sites and the amount of formazan with the exception of neuronal NADPH-d as did incubation of fresh or acetone-chloroform-pretreated sections for NADPH-d in the presence of 0.5% formaldehyde. When 1% formaldehyde or 0.5 mM permanganate were used significant amounts of formazan appeared only in central and peripheral neurons, vasal endothelial cells, small intestinal enterocytes, plasma membrane region of striated muscle fibers as well as arteriolar cells in the kidney; except for enterocytes, these observations were confirmed by NOS-immunohistochemistry which revealed in addition reactive cells in the thymus and intestinal lamina propria.
Περιγραφή
Λέξεις-κλειδιά
Acetone, Animals, Callithrix, Chloroform, Cryoultramicrotomy, Female, Fluorescent Antibody Technique, Formaldehyde, Immunohistochemistry, Male, Manganese Compounds, NADPH Dehydrogenase/*metabolism, Nitric Oxide Synthase/*immunology/*metabolism, Oxides
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/8525791
http://ac.els-cdn.com/S0065128111801972/1-s2.0-S0065128111801972-main.pdf?_tid=0a82d7691112da7185cc62706327cbb6&acdnat=1333608633_167fc28d2bf3ee90cef554a24aef0a2d
http://ac.els-cdn.com/S0065128111801972/1-s2.0-S0065128111801972-main.pdf?_tid=0a82d7691112da7185cc62706327cbb6&acdnat=1333608633_167fc28d2bf3ee90cef554a24aef0a2d
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής