Determination of glucose and fructose in mixtures by a kinetic method with chemiluminescence detection
Φόρτωση...
Ημερομηνία
Συγγραφείς
Evmiridis, N. P.
Thanasoulias, N. K.
Vlessidis, A. G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier Masson
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Analytica Chimica Acta
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
A kinetic method of analysis is described, based on oxidation by periodate for the determination of glucose and fructose in sample solutions. The method combines flow injection and chemiluminescence (CL) detection. The detection is based on the CL signal generated during oxidation of pyrogallol by periodate. The method is selective for the determination of vic-diols and polyols; the method is designed to determine hexose contents as low as 200 mu g. However, the method can be tailored for even lower amounts (10 times less). When the total hexose concentration is known, the individual fructose and glucose content can be determined from the rate constant that best-fits the kinetic data; the relative standard deviation of three independent kinetic experiments with the same ratio of concentrations was found to be within 2% and the standard error of the rate constant from the fitting treatment was found to be less than 10% in all samples of different concentration ratios between glucose and fructose. The method can be automated and controlled by computer software. Data points from two kinetic experiments with different sample quantities in the reaction mixture are required for the determination of the total hexose content and the individual fractions of glucose and fructose. (C) 1999 Elsevier Science B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
glucose/fructose, kinetic analysis, periodate oxidation, pyrogallol chemiluminescence, flow injection, liquid-phase chemiluminescence, flow-injection analysis, periodate determination, calibration curve, pyrogallol, oxidation
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000083370900007
http://ac.els-cdn.com/S0003267099004249/1-s2.0-S0003267099004249-main.pdf?_tid=7f172182-356a-11e3-ac3f-00000aab0f01&acdnat=1381821906_01362c4f55421659cda760a2cb017c81
http://ac.els-cdn.com/S0003267099004249/1-s2.0-S0003267099004249-main.pdf?_tid=7f172182-356a-11e3-ac3f-00000aab0f01&acdnat=1381821906_01362c4f55421659cda760a2cb017c81
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας