Molecular and mechanistic characterization of platelet-activating factor-like bioactivity produced upon LDL oxidation
| dc.contributor.author | Androulakis, N. | en |
| dc.contributor.author | Durand, H. | en |
| dc.contributor.author | Ninio, E. | en |
| dc.contributor.author | Tsoukatos, D. C. | en |
| dc.date.accessioned | 2015-11-24T16:42:04Z | |
| dc.date.available | 2015-11-24T16:42:04Z | |
| dc.identifier.issn | 0022-2275 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/8508 | |
| dc.rights | Default Licence | - |
| dc.subject | oxidized phospholipids | en |
| dc.subject | platelet-activating factor-acetylhydrolase | en |
| dc.subject | platelet-activating factor-transacetylase | en |
| dc.subject | mediators of inflammation | en |
| dc.subject | atherogenesis | en |
| dc.subject | low density lipoprotein | en |
| dc.subject | low-density-lipoprotein | en |
| dc.subject | paf-degrading acetylhydrolase | en |
| dc.subject | human-plasma | en |
| dc.subject | lipid-peroxidation | en |
| dc.subject | factor receptor | en |
| dc.subject | atherosclerosis | en |
| dc.subject | phosphatidylcholines | en |
| dc.subject | identification | en |
| dc.subject | phospholipids | en |
| dc.subject | cholesterol | en |
| dc.title | Molecular and mechanistic characterization of platelet-activating factor-like bioactivity produced upon LDL oxidation | en |
| heal.abstract | Oxidation of LDL is thought to be involved in both initiating and sustaining atherogenesis through the formation of proinflammatory lipids and the covalent modification of LDL particles. Platelet- activating factor (PAF; 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent phospholipid mediator involved in inflammation. Upon oxidation of LDL, oxidized phospholipids with PAF-like structure are generated, and some of them may act via the PAF receptor. We evaluated the contribution of 1-0-hexadecyl-2-acetylsn-glycero-3-phosphocholine (C16:0 PAF) and of other PAF analogs on the PAF-like bioactivity formed upon Cu2+- initiated oxidation of LDL. Reverse-phase HPLC purification and electrospray ionization-MS analyses showed that upon oxidation of LDL with inactivated PAF-acetylhydrolase (PAF-AH), C16:0 PAF accounted for > 30% of PAF-like biological activity and its sn-2 butenoyl analog accounted for > 50%. However, upon LDL oxidation in the presence of exogenous 1-0-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) without PAF-AH inactivation, C16:0 PAF formation accounted for > 90% of the biological activity recovered. We suggest that the C16:0 PAF, despite being a minor constituent of the LDL peroxidation products, may contribute substantially to the bioactivity formed in oxidized LDL. The higher bioactivity of C16:0 PAF, and the higher selectivity of the LDL-attached lyso-PAF transacetylase toward very short acyl chains [acetate (C2) vs. butanate (C4)], may explain the contribution described above. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | DOI 10.1194/jlr.M500074-JLR200 | - |
| heal.identifier.secondary | <Go to ISI>://000231244200014 | - |
| heal.identifier.secondary | http://www.jlr.org/content/46/9/1923.full.pdf | - |
| heal.journalName | Journal of Lipid Research | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2005 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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