Phospholipases A(2) and platelet-activating-factor acetylhydrolase in patients with acute respiratory distress syndrome

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dc.contributor.authorNakos, G.en
dc.contributor.authorKitsiouli, E.en
dc.contributor.authorHatzidaki, E.en
dc.contributor.authorKoulouras, V.en
dc.contributor.authorTouqui, L.en
dc.contributor.authorLekka, M. E.en
dc.date.accessioned2015-11-24T16:44:29Z
dc.date.available2015-11-24T16:44:29Z
dc.identifier.issn0090-3493-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8808
dc.rightsDefault Licence-
dc.subjectphospholipase a(2)en
dc.subjectplatelet-activating-factor acetylhydrolaseen
dc.subjectacute respiratory distress syndromeen
dc.subjectacute lung injuryen
dc.subjectfluorescenceen
dc.subjectacute lung injuryen
dc.subjectmultiple-organ failureen
dc.subjectsurfactant protein-aen
dc.subjectbronchoalveolar lavageen
dc.subjectclinical-trialen
dc.subjectsevere sepsisen
dc.subjectpulmonary surfactanten
dc.subjectii phospholipase-a2en
dc.subjectoleic-aciden
dc.subjecta(2)en
dc.titlePhospholipases A(2) and platelet-activating-factor acetylhydrolase in patients with acute respiratory distress syndromeen
heal.abstractObjective. Phospholipases A(2) (PLA(2)) comprise a family of enzymes probably implicated in the development of acute respiratory distress syndrome (ARDS). The aim was to investigate PLA2 activities and characteristics in bronchoalveolar lavage (BAL) fluid, BAL cells, and plasma from patients with ARDS by a fluorometric method. Design: Prospective, controlled study. Setting: Fourteen-bed polyvalent intensive care unit in a university hospital. Patients: A total of 31 mechanically ventilated patients, 20 with and 11 without ARDS, were studied. Intervention. BAL was performed by fiberoptic bronchoscopy in mechanically ventilated patients with a controlled mechanical ventilation mode. Measurements: PLA(2) and platelet-activating-factor acetylhydrolase were determined in BAL fluid, cells, and plasma. For the classification of PLA(2)-specific inhibitors, Western blot analysis and their biochemical characteristics were used. Results: In ARDS patients, increased PLA(2) levels were detected in BAL fluid, BAL cells, and plasma compared with the control patients. PLA(2) in BAL fluid was mainly type IIA secretory and cytosolic types. In plasma, type IIA secretory and cytosolic and a Ca2+-independent PLA(2) were found. In BAL cells, a cytosolic form, probably a Ca2+-independent intracellular form, and a low activity of type IIA secretory PLA(2) was also observed. Total PLA(2) activity correlated inversely with Pao(2)/Fio(2) ratio and positively with the mortality rate. Patients with direct ARDS exhibited higher PLA(2) activity compared with patients with indirect ARDS. Platelet-activating-factor acetylhydrolase activity was higher in BAL fluid and plasma, but it was lower in BAL cells. Conclusion. Ca2+-dependent secretory, cytosolic, and Ca2+-independent forms of PLA(2) and platelet-activating-factor acetylhydrolase could play important roles in the development or down-regulation of inflammation in ARDS, respectively.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDoi 10.1097/01.Ccm.0000158519.80090.74-
heal.identifier.secondary<Go to ISI>://000228282700012-
heal.journalNameCrit Care Meden
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2005-
heal.publisherLippincott, Williams & Wilkinsen
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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