Endogenous ACh effects on NMDA-induced interictal-like discharges along the septotemporal hippocampal axis of adult rats and their modulation by an early life generalized seizure
Φόρτωση...
Ημερομηνία
Συγγραφείς
Mikroulis, Apostolos V.
Psarropoulou, Caterina
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Blackwell Publishing Ltd
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Epilepsia
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Purpose: Our earlier findings of the modulation of cholinergic neurotransmission by an early life generalized seizure and the reported interaction between muscarinic and N-methyl-d-aspartate (NMDA) receptors prompted us to investigate the effects of endogenous acetylcholine (ACh) on the frequency (Hz) of the epileptiform discharges following NMDA-receptor activation in the hippocampal slice. Methods: A sustained (>20Β min) generalized convulsion was induced in Sprague-Dawley juvenile rats by intraperitoneal injection with pentylenetetrazole (PTZ, 70-90Β mg/kg) at postnatal day (P) 20. Temporal and septal hippocampal slices were prepared of normal (N) and PTZ-treated (PTZ) adult (β ₯P60) rats, and CA3 field potentials were recorded during perfusion with Mg2+-free artificial cerebrospinal fluid (ACSF) or with ACSF containing 50Β ΞΌm 4-aminopyridine (4-AP). Key Findings: In Mg2+-free ACSF, spontaneous interictal-like epileptiform discharges (IEDs) were recorded in all slices, with significantly higher frequencies in temporal (0.46Β Β±Β 0.03Β Hz, nΒ =Β 85) versus septal slices (0.20Β Β± 0.02Β Hz, nΒ =Β 47, pΒ <Β 0.000001) but no consistent differences in any other group (i.e., male vs. female or N vs. PTZ). The anticholinesterase eserine (10Β ΞΌm) increased their frequencies by 150-200% in N-septal and in all temporal slices and by 300% in PTZ-septal slices (pΒ =Β 0.0028). In 60% of the slices the excitatory effect persisted throughout drug perfusion, whereas in the remaining ones it was distinguished in two phases: an early " transient" and a late " steady state." The steady-state frequencies resembled the predrug ones in N slices but remained significantly elevated in PTZ slices, especially in the septal group. The muscarinic antagonist atropine (1Β ΞΌm) decreased IED frequency in all slices (nΒ =Β 36, pΒ =Β 0.005) and also fully reversed the eserine effect (nΒ =Β 38, pΒ <Β 0.0001). In 4-AP ACSF, eserine increased spontaneous IED frequency (nΒ =Β 21) in N and PTZ slices alike; IEDs were subsequently abolished by addition of the NMDA-receptor antagonist D(β )-2-amino-5-phosphonopentanoic acid (AP5; 50Β ΞΌm, nΒ =Β 6). Significance: These results demonstrate an intrinsic tonic positive muscarinic acetylcholine receptor (mAChR) contribution to the frequency of NMDA receptor-dependent epileptiform discharges that is amplified following an elevation of endogenous ACh and is more pronounced in the septal hippocampus. Moreover, this positive mAChR contribution to the frequency of IEDs is even more pronounced and persistent in the septal extremity after an early life generalized sustained convulsion. This cholinergic enhancement of the excitatory septal hippocampal output may influence cognitive function and performance, and possibly the adult seizure threshold.
Περιγραφή
Λέξεις-κλειδιά
Brain, Seizures, NMDA receptors, Spontaneous discharges, Muscarinic receptors, Eserine, Atropine, Epileptiform discharges, Interictal
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://dx.doi.org/10.1111/j.1528-1167.2012.03440.x
http://onlinelibrary.wiley.com/store/10.1111/j.1528-1167.2012.03440.x/asset/j.1528-1167.2012.03440.x.pdf?v=1&t=hfxntnfu&s=47927d4cea7c919387cf9f2f8f1e678b2a14f720
http://onlinelibrary.wiley.com/store/10.1111/j.1528-1167.2012.03440.x/asset/j.1528-1167.2012.03440.x.pdf?v=1&t=hfxntnfu&s=47927d4cea7c919387cf9f2f8f1e678b2a14f720
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών