Selection of single-stranded deoxyribonucleic acid spermatozoa via the SpermPrep filtration column
| dc.contributor.author | Sofikitis, N. | en |
| dc.contributor.author | Miyagawa, I. | en |
| dc.contributor.author | Zavos, P. M. | en |
| dc.date.accessioned | 2015-11-24T18:52:38Z | |
| dc.date.available | 2015-11-24T18:52:38Z | |
| dc.identifier.issn | 0015-0282 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/18420 | |
| dc.rights | Default Licence | - |
| dc.subject | DNA, Single-Stranded/*analysis | en |
| dc.subject | Filtration | en |
| dc.subject | Humans | en |
| dc.subject | Male | en |
| dc.subject | Sperm Motility | en |
| dc.subject | Spermatozoa/*chemistry | en |
| dc.title | Selection of single-stranded deoxyribonucleic acid spermatozoa via the SpermPrep filtration column | en |
| heal.abstract | Eighteen semen samples were collected from 18 normospermic men. Two aliquots (1 mL) were prepared from each ejaculate, washed with Ham's F-10, and each washed sperm pellet was reconstituted in 2 mL volume of Ham's F-10 medium. Each aliquot one was stained using the AO-staining method. Each aliquot two was filtered via the SpermPrep II method, and the recovered spermatozoa were stained similarly. The proportion of single-stranded DNA (red) spermatozoa to double-stranded (green) spermatozoa was significantly higher in aliquot one than in the postfiltered sample (aliquot two), suggesting that the SpermPrep filtration procedure selectively entrapped the spermatozoa with abnormal DNA. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/8458481 | - |
| heal.journalName | Fertil Steril | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1993 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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