Butyrate enhances the synthesis of interphotoreceptor retinoid-binding protein (IRBP) by Y-79 human retinoblastoma cells

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dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorWiggert, B.en
dc.contributor.authorLee, L.en
dc.contributor.authorChader, G. J.en
dc.date.accessioned2015-11-24T19:32:05Z
dc.date.available2015-11-24T19:32:05Z
dc.identifier.issn0021-9541-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23362
dc.rightsDefault Licence-
dc.subjectAmino Acids/metabolismen
dc.subjectButyrates/*pharmacologyen
dc.subjectButyric Aciden
dc.subjectButyric Acids/*pharmacologyen
dc.subjectCell Lineen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subject*Eye Proteinsen
dc.subjectFluorescent Antibody Techniqueen
dc.subjectGlucosamine/metabolismen
dc.subjectHistocytochemistryen
dc.subjectHumansen
dc.subjectLectinsen
dc.subjectMicroscopy, Phase-Contrasten
dc.subjectMonensin/pharmacologyen
dc.subjectRetinoblastoma/*metabolismen
dc.subjectRetinol-Binding Proteins/*biosynthesisen
dc.subjectTime Factorsen
dc.subjectWheat Germ Agglutininsen
dc.titleButyrate enhances the synthesis of interphotoreceptor retinoid-binding protein (IRBP) by Y-79 human retinoblastoma cellsen
heal.abstractThe synthesis and secretion of interphotoreceptor retinoid-binding protein (IRBP) from Y-79 human retinoblastoma cells was investigated using immunocytochemistry and SDS-polyacrylamide gel electrophoresis. Indirect immunofluorescence of cells growing in monolayer culture for 11 and 13 days showed no significant IRBP staining although by SDS-polyacrylamide gel electrophoresis, a small amount of IRBP was detected in the culture medium, suggesting synthesis and extracellular secretion. Butyrate (2mM) treatment of cells starting on the eighth day of culture resulted in a dramatic increase of IRBP fluorescence 3-5 days after treatment. Treatment of cells in all conditions with 1 microM monensin for 3 h showed concentration of IRBP in the Golgi apparatus of about 10-20% of cells as proved by a double immunofluorescent technique, employing anti-IRBP antibody and wheat-germ agglutinin. Incubation of cells with either radiolabeled amino acids or glucosamine followed by analysis of cell cytosol and culture medium by SDS-polyacrylamide gel electrophoresis also confirmed that 1) IRBP is synthesized by the Y-79 cells and secreted into the medium and 2) its production is markedly increased by butyrate treatment. The enhancement of IRBP synthesis by butyrate suggests biochemical differentiation of Y-79 cells possibly into photoreceptor-like cells and offers a new system for studying the properties of this unique retinoid-binding protein and of factors that control its synthesis and secretion.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1002/jcp.1041240210-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/3900095-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/jcp.1041240210/asset/1041240210_ftp.pdf?v=1&t=h0b2ibkr&s=4f8ce16300f6a70744949825459e0b36c93a42d8-
heal.journalNameJ Cell Physiolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1985-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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