Localization of an alkyl-acetyl-glycerol-CDP-choline : cholinephosphotransferase activity in submitochondrial fractions of Tetrahymena pyriformis
Φόρτωση...
Ημερομηνία
Συγγραφείς
Tellis, C.
Pantazi, D.
Ioachim, E.
Galani, V.
Lekka, M. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Eur J Cell Biol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Tetrahymena pyriformis contains platelet-activating factor (PAF) as a minor lipid, which is biosynthesized de novo. A dithiothreitoll-insensitive CDP-choline:cholinephospotransferase (AAG-CPT), which utilizes alkyl-acetyl-glycerol as a substrate, had been detected in both the mitochondrial and microsomal fractions of the protozoan. In the present report, localization of this enzyme in submitochondrial fractions was studied. Cell fractionation was evaluated with enzyme and morphological markers. In this respect, succinate dehydrogenase, NADPH:cytochrome c reductase, glucose-6-phosphatase, alkaline phosphatase, monoaminoxidase, and cytochrome c oxidase activities were investigated. In the presence of antimycin A, mitochondrial activity of NADPH-cytochrome c reductase, was increased, while the microsomal one was reduced. Cardiolipin was distributed in the inner mitochondrial membrane. Alkaline phosphatase was found exclusively in the cytosol of the protozoan. The main portion of the dithiothreitoll-insensitive AAG-CPT was localized in the inner mitochondrial membrane. Our data indicate that mitochondria are able to produce PAF, which might be associated with their function.
Περιγραφή
Λέξεις-κλειδιά
tetrahymena pyriformis, nadph-cytochrome c reductase, succinate dehydrogenase, paf, cholinephosphotransferase, platelet-activating-factor, cytochrome-c reductase, mitochondria, membrane, stimulation, enzymes, cells, acid, paf
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000187637800005
http://ac.els-cdn.com/S0171933504703295/1-s2.0-S0171933504703295-main.pdf?_tid=67aaa81651fe01a401302d5e7dd61334&acdnat=1333034084_4a72ea5b60e8dff0442d232d830916ea
http://ac.els-cdn.com/S0171933504703295/1-s2.0-S0171933504703295-main.pdf?_tid=67aaa81651fe01a401302d5e7dd61334&acdnat=1333034084_4a72ea5b60e8dff0442d232d830916ea
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας