Ozone Monitoring Based on a Biosensor Concept Utilizing a Reagentless Alcohol Oxidase Electrode
Φόρτωση...
Ημερομηνία
Συγγραφείς
D.V. Stergiou,
M.I. Prodromidis,
P.G. Veltsistas,
N. Evmiridis
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
American Chemical Society
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Anal Chem
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
An electrochemical method based on the concept of a biosensor for the monitoring of ozone is described for first time. The proposed method includes two parts:"‰ a selective sorbent for ozone, that is, eugenol, and a formaldehyde amperometric biosensor mounted into a flow-through cell. Ozone adds rapidly to the double bond of the allyl group of eugenol, which has been immobilized onto a hydrophobic C-18 reactor and the so produced formaldehyde is collected into the working buffer solution (sampler) and pumped to the detector. A multimembrane assembly consisting of an alcohol oxidase-modified nylon membrane sandwiched between an outer polycarbonate and an inner cellulose acetate membrane was fitted onto a Pt electrode and the enzymatically produced H2O2 was monitored at +0.65 V (vs Ag/AgCl/KCl 3 M). Under optimum conditions, a linear calibration curve over the concentration range 3β�’200 ΞΌgΒ·mL-1 ozone was constructed. The detection limit (S/N = 3) was calculated at 1.1 ΞΌgΒ·mL-1 ozone. The proposed method is interference-free from other gases such as O2, Ar, N2, N2O, NOCl, SO2, NH3, and CO2, which were tested at concentrations >200-fold higher than that of 100 ΞΌgΒ·mL-1 ozone used for comparison. Besides selectivity, the method is easy to perform and reproducible; its applicability in synthetic gaseous samples is also demonstrated.
Περιγραφή
Λέξεις-κλειδιά
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://pubs.acs.org/doi/abs/10.1021/ac060230h
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας