Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia

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dc.contributor.authorYamanaka, K.en
dc.contributor.authorSofikitis, N. V.en
dc.contributor.authorMiyagawa, I.en
dc.contributor.authorYamamoto, Y.en
dc.contributor.authorToda, T.en
dc.contributor.authorAntypas, S.en
dc.contributor.authorDimitriadis, D.en
dc.contributor.authorTakenaka, M.en
dc.contributor.authorTaniguchi, K.en
dc.contributor.authorTakahashi, K.en
dc.contributor.authorTsukamoto, S.en
dc.contributor.authorKawamura, H.en
dc.contributor.authorNeil, M.en
dc.date.accessioned2015-11-24T19:00:51Z
dc.date.available2015-11-24T19:00:51Z
dc.identifier.issn1058-0468-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19606
dc.rightsDefault Licence-
dc.subjectAdulten
dc.subjectCell Nucleusen
dc.subjectCells, Cultureden
dc.subjectEmbryo Transfer/statistics & numerical dataen
dc.subjectFemaleen
dc.subjectFertilization in Vitro/*methodsen
dc.subjectHumansen
dc.subjectMaleen
dc.subject*Microinjectionsen
dc.subjectMicroscopy, Confocalen
dc.subjectMicroscopy, Electronen
dc.subjectOligospermia/*therapyen
dc.subjectOocytes/physiologyen
dc.subjectPregnancyen
dc.subjectSpermatids/*physiology/ultrastructureen
dc.subjectTreatment Outcomeen
dc.titleOoplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermiaen
heal.abstractPURPOSE: Our objective was to apply ooplasmic round spermatid nuclear injections for the treatment of nonobstructive azoospermia. MATERIALS: Participants were nine azoospermic men who had previously undergone diagnostic testicular biopsy. Spermatogenetic arrest was diagnosed at the round spermatid stage (n = 6) or primary spermatocyte stage (n = 3). A second (therapeutic) testicular biopsy was performed and round spermatid nuclei were recovered from all the participants. RESULTS: Forty-nine mature oocytes were successfully injected with nuclei and then cultured for 72 hr. Twenty-four embryos were transferred to nine women. No pregnancy was achieved. CONCLUSIONS: Round spermatids can be recovered from therapeutic testicular biopsy material of men negative for round spermatids in previous routine diagnostic testicular biopsy specimens. Round spermatid nuclear injections may play a role in the treatment of nonobstructive azoospermia.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9013313-
heal.journalNameJ Assist Reprod Geneten
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1997-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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