Bcl-2 affects survival but not neuronal differentiation of PC12 cells
| dc.contributor.author | Batistatou, A. | en |
| dc.contributor.author | Merry, D. E. | en |
| dc.contributor.author | Korsmeyer, S. J. | en |
| dc.contributor.author | Greene, L. A. | en |
| dc.date.accessioned | 2015-11-24T19:33:22Z | |
| dc.date.available | 2015-11-24T19:33:22Z | |
| dc.identifier.issn | 0270-6474 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23529 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Blotting, Northern | en |
| dc.subject | Blotting, Western | en |
| dc.subject | Cell Differentiation/drug effects/*physiology | en |
| dc.subject | Cell Survival/drug effects/*physiology | en |
| dc.subject | Humans | en |
| dc.subject | Kinetics | en |
| dc.subject | Nerve Growth Factors/pharmacology | en |
| dc.subject | Neurons/*cytology/metabolism | en |
| dc.subject | PC12 Cells | en |
| dc.subject | Protein-Tyrosine Kinases/metabolism | en |
| dc.subject | Proto-Oncogene Proteins/analysis/biosynthesis/*metabolism | en |
| dc.subject | Proto-Oncogene Proteins c-bcl-2 | en |
| dc.subject | *Proto-Oncogenes | en |
| dc.subject | RNA/analysis | en |
| dc.subject | RNA, Messenger/biosynthesis/metabolism | en |
| dc.subject | Recombinant Proteins/analysis/biosynthesis/metabolism | en |
| dc.subject | Time Factors | en |
| dc.subject | Transfection | en |
| dc.title | Bcl-2 affects survival but not neuronal differentiation of PC12 cells | en |
| heal.abstract | Past studies have shown that serum-free cultures of PC12 cells are a useful model system for studying the mechanisms of neuronal death after neurotrophic factor deprivation. These cultures, as well as NGF-deprived cultures of sympathetic neurons, manifest and endonuclease activity that leads to "apoptotic" internucleosomal DNA cleavage. Overexpression of the proto-oncogene bcl-2 blocks apoptotic death in various cell types. To study the actions of this protein in neuronal cells, we derived PC12 cell lines stably transfected with a cDNA encoding human bcl-2. It is reported here that lines expressing high levels of the exogenous bcl-2 protein are protected from both death and apoptotic DNA fragmentation caused by removal of trophic support. However, expression of high levels of exogenous bcl-2 neither mimics nor interferes with promotion of neurite outgrowth by NGF. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/7692014 | - |
| heal.journalName | J Neurosci | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1993 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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