A revisit to the retention mechanism of hydrophilic interaction liquid chromatography using model organic compounds
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Ημερομηνία
Συγγραφείς
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Chromatography A
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
In this work, a revisit to the retention mechanism of HILIC was attempted to point out critical factors that contribute to the chromatographic regime as well as to bring out subtle details of the relative contribution of partitioning and surface adsorption. In this vein, the retention behaviour of a set of water-soluble vitamins (WSVs) and toluene on three silica based columns was evaluated under varying chromatographic conditions. The data obtained were associated with the hydration degree of the stationary phases and the ability of the organic solvents to disrupt the formation of the water-enriched layer. Moreover, the elution behaviour of toluene at different buffer salt concentrations in the mobile phase, confirmed the preferential partition of salt ions into the stagnant layer, as ACN content was increased. The results from the fitting of partitioning and surface adsorption models indicated differences in the contribution of the two retention mechanisms to both neutral and charged compounds. The occurrence of surface adsorption and the retentivity differences for neutral WSVs depend on the hydration degree and the hydrogen bonding properties of the solutes and the column surface, respectively. For charged solutes experiencing electrostatic repulsion, the contribution of the adsorption mechanism at highly organic mobile phases, emanates from both the weak effect of buffer salt ions on the electrostatic interaction and the strong effect of hydrophilic interactions. On the other hand, the chromatographic retention of electrostatically attracted solutes indicates that the surface adsorption dominates, even at mobile phases rich in water. (C) 2011 Elsevier B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
hydrophilic interaction liquid chromatography (hilic), stationary phases, polar organic solvents, retention mechanism, water-soluble vitamins, toluene, high-purity silica, stationary phases, polar compounds, basic compounds, mobile-phase, diol column, separation, behavior, water, temperature
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000291065500007
http://ac.els-cdn.com/S0021967311003141/1-s2.0-S0021967311003141-main.pdf?_tid=b2b238cb154da5f5a4a4491e5ef4b750&acdnat=1333040825_fd395166bae009eabd7a15774c90b07a
http://ac.els-cdn.com/S0021967311003141/1-s2.0-S0021967311003141-main.pdf?_tid=b2b238cb154da5f5a4a4491e5ef4b750&acdnat=1333040825_fd395166bae009eabd7a15774c90b07a
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας