A technique for the measurement of orthophosphate in human erythrocytes, and some studies of its determinants
Loading...
Date
Authors
Challa, A.
Bevington, A.
Angier, C. M.
Asbury, A. J.
Preston, C. J.
Russell, R. G.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Type
Type of the conference item
Journal type
peer-reviewed
Educational material type
Conference Name
Journal name
Clin Sci (Lond)
Book name
Book series
Book edition
Alternative title / Subtitle
Description
A technique is described for the determination of orthophosphate (Pi) in human erythrocytes. The advantages of the technique are that it uses whole blood rather than separated erythrocytes, that it avoids major hydrolysis of organic phosphates, that it takes account of incomplete recovery of Pi and that it minimizes the effects of chilling the cells. In chilled samples from 46 patients in an intensive care unit, the cellular concentration of Pi was proportional to that in plasma. Blood samples from nine normal subjects were incubated at 37 degrees C. The cellular Pi was 0.79 mmol/litre of cells using an external standardization and 0.67 using an internal standardization. When the same cell samples were chilled on ice for 30 min, the internally standardized value decreased further to 0.57 mmol/litre of cells. These results suggest that differences in recovery, and the extent of chilling, contribute to the variability in the previously reported values for erythrocyte Pi. If Pi, like chloride, had distributed passively between cells and plasma in these samples, the cell to plasma molar concentration ratio for Pi should have been 0.29, compared with the measured value of 0.64. This difference suggests that some factor, in addition to passive diffusion, determined the distribution of Pi.
Description
Keywords
Cold Temperature, Erythrocytes/*metabolism, Hematocrit, Humans, Methods, Phosphates/*blood
Subject classification
Citation
Link
http://www.ncbi.nlm.nih.gov/pubmed/4042544
Language
en
Publishing department/division
Advisor name
Examining committee
General Description / Additional Comments
Institution and School/Department of submitter
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής