Identification of distinct SET/TAF-Ibeta domains required for core histone binding and quantitative characterisation of the interaction
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Karetsou, Z.
Emmanouilidou, A.
Sanidas, I.
Liokatis, S.
Nikolakaki, E.
Politou, A. S.
Papamarcaki, T.
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peer-reviewed
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BMC Biochem
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BACKGROUND: The assembly of nucleosomes to higher-order chromatin structures is finely tuned by the relative affinities of histones for chaperones and nucleosomal binding sites. The myeloid leukaemia protein SET/TAF-Ibeta belongs to the NAP1 family of histone chaperones and participates in several chromatin-based mechanisms, such as chromatin assembly, nucleosome reorganisation and transcriptional activation. To better understand the histone chaperone function of SET/TAF-Ibeta, we designed several SET/TAF-Ibeta truncations, examined their structural integrity by circular Dichroism and assessed qualitatively and quantitatively the histone binding properties of wild-type protein and mutant forms using GST-pull down experiments and fluorescence spectroscopy-based binding assays. RESULTS: Wild type SET/TAF-Ibeta binds to histones H2B and H3 with Kd values of 2.87 and 0.15 microM, respectively. The preferential binding of SET/TAF-Ibeta to histone H3 is mediated by its central region and the globular part of H3. On the contrary, the acidic C-terminal tail and the amino-terminal dimerisation domain of SET/TAF-Ibeta, as well as the H3 amino-terminal tail, are dispensable for this interaction. CONCLUSION: This type of analysis allowed us to assess the relative affinities of SET/TAF-Ibeta for different histones and identify the domains of the protein required for effective histone recognition. Our findings are consistent with recent structural studies of SET/TAF-Ibeta and can be valuable to understand the role of SET/TAF-Ibeta in chromatin function.
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Amino Acid Sequence, Chromatin/metabolism, Chromosomal Proteins, Non-Histone/chemistry/*metabolism, Circular Dichroism, Histone Chaperones, Histones/*metabolism, Molecular Chaperones/metabolism, Molecular Sequence Data, Mutant Proteins/metabolism, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins/metabolism, Spectrometry, Fluorescence, Transcription Factors/chemistry/*metabolism
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http://www.ncbi.nlm.nih.gov/pubmed/19358706
http://www.biomedcentral.com/1471-2091/10/10
http://www.biomedcentral.com/1471-2091/10/10
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής