Cell-free DNA and RNA in plasma as a new molecular marker for prostate and breast cancer

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dc.contributor.authorPapadopoulou, E.en
dc.contributor.authorDavilas, E.en
dc.contributor.authorSotiriou, V.en
dc.contributor.authorGeorgakopoulos, E.en
dc.contributor.authorGeorgakopoulou, S.en
dc.contributor.authorKoliopanos, A.en
dc.contributor.authorAggelakis, F.en
dc.contributor.authorDardoufas, K.en
dc.contributor.authorAgnanti, N. J.en
dc.contributor.authorKarydas, I.en
dc.contributor.authorNasioulas, G.en
dc.date.accessioned2015-11-24T19:31:18Z
dc.date.available2015-11-24T19:31:18Z
dc.identifier.issn0077-8923-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23243
dc.rightsDefault Licence-
dc.subjectAntigens, Surface/blood/geneticsen
dc.subject*Breast Neoplasms/blood/diagnosis/geneticsen
dc.subjectCarcinoembryonic Antigen/blood/geneticsen
dc.subjectCell Cycle Proteins/blood/geneticsen
dc.subjectDNA/*blooden
dc.subjectDNA Methylationen
dc.subjectDNA-Binding Proteins/blood/geneticsen
dc.subjectFemaleen
dc.subjectGenetic Markersen
dc.subjectGlutamate Carboxypeptidase II/blood/geneticsen
dc.subjectGlutathione S-Transferase pi/blood/geneticsen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectPlasma/*chemistryen
dc.subjectPromoter Regions, Geneticen
dc.subject*Prostatic Neoplasms/blood/diagnosis/geneticsen
dc.subjectProtein-Serine-Threonine Kinases/blood/geneticsen
dc.subjectRNA/*blooden
dc.subject*Tumor Markers, Biologicalen
dc.subjectTumor Suppressor Proteins/blood/geneticsen
dc.titleCell-free DNA and RNA in plasma as a new molecular marker for prostate and breast canceren
heal.abstractIn this study, we examined several molecular markers in prostate and breast cancer patients and in normal individuals. The markers tested were: variations in the quantity of plasma DNA, glutathione-S-transferase P1 gene (GSTP1), Ras association domain family 1A (RASSF1A), and ataxia telangiectasia mutated (ATM) methylation status in plasma, carcinoembryonic antigen (CEA) and prostate-specific membrane antigen (PSMA) mRNA in peripheral blood mononuclear cells (PBMC) and plasma samples from prostate cancer patients. DNA quantification in plasma was performed using real-time PCR (RT-PCR). We assessed the methylation status of GSTP1 in plasma DNA using methylation-specific PCR (MSP) assay, while the methylation status of RASSF1A and ATM genes was examined by the MethyLight technology. RT-PCR analysis was used for the detection of mRNA, PSMA, and CEA. In 58.3% of newly diagnosed prostate cancer patients and 26.7% of prostate cancer patients under therapy, plasma DNA levels were increased. Additionally, 48.5% of breast cancer patients showed plasma DNA levels above the cutoff limit. GSTP1 Promotor hypermethylation was detectable in 75% of plasma samples obtained from patients with newly diagnosed prostate cancer and in 36.8% of patients under therapy, whereas 26% and 14% of the breast cancer patients tested were positive for RASSF1A and ATM methylation, respectively. The combination of DNA load and promotor methylation status identified 88% of prostate cancer patients and 54% of breast cancer patients. This study shows that free-circulating DNA can be detected in cancer patients compared with disease-free individuals, and suggests a new, noninvasive approach for early detection of cancer.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1196/annals.1368.032-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/17108217-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1196/annals.1368.032/asset/annals.1368.032.pdf?v=1&t=h1sz13hz&s=6096b5bb41fe7a85a2e431061e077ad6968197f7-
heal.journalNameAnn N Y Acad Scien
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2006-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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