Determination of okadaic acid, dinophysistoxin-1 and related esters in Greek mussels using HPLC with fluorometric detection, LC-MS/MS and mouse bioassay
Φόρτωση...
Ημερομηνία
Συγγραφείς
Louppis, A. P.
Badeka, A. V.
Katikou, P.
Paleologos, E. K.
Kontominas, M. G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Toxicon
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
An approach involving both chemical and biological methods was undertaken for the detection and quantification of the marine toxins okadaic acid (OA), dinophysistoxin-1 (DTX-1) and their respective esters in mussels from different sampling sites in Greece during the period 2006-2007. Samples were analyzed by means of a) high performance liquid chromatography with fluorometric detection (HPLC-FLD), using 9-athryldiazomethane (ADAM), as a pre-column derivatization reagent, b) liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and c) the mouse bioassay. Free OA and DTX-1 were determined by both HPLC-FLD and LC-MS/MS, while their respective esters were determined only by LC-MS/MS after alkaline hydrolysis of the samples. The detection limit (L.O.D.) and quantification limit (L.O.Q) of the HPLC-FLD method were 0.015 mu g/g HP and 0.050 mu g/g HP, respectively, for OA. The detection limit (L.O.D.) and quantification limit (L.O.Q.) of the LC-MS/MS method were 0.045 mu g/g HP and 0.135 mu g/g HP, respectively, for OA. Comparison of results between the two analytical methods showed excellent agreement (100%), while both HPLC-FLD and LC-MS/MS methods showed an agreement of 97.1% compared to the mouse bioassay. (C) 2009 Elsevier Ltd. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
diarrhetic shellfish poisoning, okadaic acid, dinophysistoxins, esters of okadaic acid, mussels, mouse bioassay, hplc, lc-ms/ms, chromatography-mass-spectrometry, diarrhetic shellfish toxins, performance liquid-chromatography, lipophilic toxins, marine toxins, portuguese bivalves, poisoning toxins, thermaikos gulf, fatty-acids, dsp toxins
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000275705300006
http://ac.els-cdn.com/S0041010109005157/1-s2.0-S0041010109005157-main.pdf?_tid=716dfd9b8dc7d15b76a0c42a9f0bc3cc&acdnat=1333025075_b904f6133e717da2898f307e2ece7ba5
http://ac.els-cdn.com/S0041010109005157/1-s2.0-S0041010109005157-main.pdf?_tid=716dfd9b8dc7d15b76a0c42a9f0bc3cc&acdnat=1333025075_b904f6133e717da2898f307e2ece7ba5
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας