Adenovirus-mediated transfer of the p53 gene produces rapid and generalized death of human glioma cells via apoptosis

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dc.contributor.authorGomez-Manzano, C.en
dc.contributor.authorFueyo, J.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorSteck, P. A.en
dc.contributor.authorRoth, J. A.en
dc.contributor.authorMcDonnell, T. J.en
dc.contributor.authorSteck, K. D.en
dc.contributor.authorLevin, V. A.en
dc.contributor.authorYung, W. K.en
dc.date.accessioned2015-11-24T19:37:34Z
dc.date.available2015-11-24T19:37:34Z
dc.identifier.issn0008-5472-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24049
dc.rightsDefault Licence-
dc.subject*Adenoviruses, Humanen
dc.subject*Apoptosisen
dc.subjectBlotting, Westernen
dc.subjectCell Divisionen
dc.subjectCell Lineen
dc.subjectFlow Cytometryen
dc.subjectGene Expressionen
dc.subject*Genes, p53en
dc.subjectGenetic Vectorsen
dc.subjectGlioma/geneticsen
dc.subjectHomozygoteen
dc.subjectHumansen
dc.subjectKineticsen
dc.subjectRecombinant Proteins/biosynthesisen
dc.subjectTime Factorsen
dc.subjectTransfection/*methodsen
dc.subjectTumor Cells, Cultureden
dc.subjectTumor Suppressor Protein p53/*biosynthesisen
dc.titleAdenovirus-mediated transfer of the p53 gene produces rapid and generalized death of human glioma cells via apoptosisen
heal.abstractWild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U-373 MG, and A-172) expressed endogenous mutant p53, and the other three (U-87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8630997-
heal.journalNameCancer Resen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1996-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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