Molecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia

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dc.contributor.authorTzavaras, T.en
dc.contributor.authorStewart, M.en
dc.contributor.authorMcDougall, A.en
dc.contributor.authorFulton, R.en
dc.contributor.authorTesta, N.en
dc.contributor.authorOnions, D. E.en
dc.contributor.authorNeil, J. C.en
dc.date.accessioned2015-11-24T19:04:06Z
dc.date.available2015-11-24T19:04:06Z
dc.identifier.issn0022-1317-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19970
dc.rightsDefault Licence-
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectBlotting, Southernen
dc.subjectCat Diseases/*microbiologyen
dc.subjectCatsen
dc.subjectCloning, Molecularen
dc.subjectDNA Replicationen
dc.subjectDNA, Viral/analysis/biosynthesis/geneticsen
dc.subjectDefective Viruses/*genetics/physiologyen
dc.subjectGenes, gagen
dc.subjectLeukemia Virus, Feline/*genetics/physiologyen
dc.subjectLeukemia, Myeloid/microbiology/*veterinaryen
dc.subjectMolecular Sequence Dataen
dc.subjectNucleic Acid Hybridizationen
dc.subjectProviruses/*genetics/physiologyen
dc.subjectRestriction Mappingen
dc.subjectTransfectionen
dc.subjectVirus Replicationen
dc.titleMolecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemiaen
heal.abstractThe GM1 strain of feline leukaemia virus (FeLV) was isolated from a naturally occurring case of myeloid leukaemia and induces severe haematopoietic abnormalities, including myeloblastic leukaemia, on inoculation into cats. Molecular clones of FeLV-GM1 proviruses were obtained and studied by restriction enzyme mapping, blot hybridization and partial DNA sequence analysis. Two types of clone were isolated; the first was a replication-competent FeLV of subgroup A, resembling other low or minimally pathogenic FeLV-A isolates; the second was replication-defective with extensive deletions and mutations in gag and pol, although it has an intact env gene of subgroup B phenotype. Large segments of the defective proviruses, from the 5' leader sequence upstream of the gag gene to the 5' half of the env gene, show structural hallmarks of endogenous FeLV-related proviruses. Infectious FeLV-GM1 viruses recovered after transfection were tested for their leukaemogenic potential in newborn cats. Early polyclonal myeloproliferative changes were observed in cats inoculated with FeLV-A/GM1 alone, although these were more pronounced in animals receiving the full FeLV-AB/GM1 complex reconstituted by cotransfection of the defective virus FeLV-B with its FeLV-A helper. Analysis of viruses in the bone marrow showed that replication of the subgroup B component is delayed and restricted to a proportion of cats. Most of the infected cats developed persistent abnormalities of haematopoiesis and one progressed to disseminated myeloid leukaemia. The defective recombinant FeLV-B/GM1 appears to play an indirect but important role in myeloid leukaemogenesis.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/2155287-
heal.journalNameJ Gen Virolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1990-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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