The Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanisms

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dc.contributor.authorSchnatwinkel, C.en
dc.contributor.authorChristoforidis, S.en
dc.contributor.authorLindsay, M. R.en
dc.contributor.authorUttenweiler-Joseph, S.en
dc.contributor.authorWilm, M.en
dc.contributor.authorParton, R. G.en
dc.contributor.authorZerial, M.en
dc.date.accessioned2015-11-24T19:33:07Z
dc.date.available2015-11-24T19:33:07Z
dc.identifier.issn1545-7885-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23499
dc.rightsDefault Licence-
dc.subjectAdenoviridae/geneticsen
dc.subjectAndrostadienes/pharmacologyen
dc.subjectAnimalsen
dc.subjectCell Lineen
dc.subjectCell Line, Tumoren
dc.subjectCell Membrane/metabolismen
dc.subjectCloning, Molecularen
dc.subjectDogsen
dc.subjectDown-Regulationen
dc.subjectEndocytosis/*physiologyen
dc.subjectEndosomes/metabolismen
dc.subjectEnzyme Inhibitors/pharmacologyen
dc.subjectEpithelial Cells/cytologyen
dc.subjectFibroblasts/metabolismen
dc.subjectGTP Phosphohydrolases/chemistryen
dc.subject*Gene Expression Regulationen
dc.subjectHumansen
dc.subjectInositol Phosphates/chemistryen
dc.subjectIntracellular Signaling Peptides and Proteins/genetics/*physiologyen
dc.subjectLiposomes/metabolismen
dc.subjectMiceen
dc.subjectMicroscopy, Confocalen
dc.subjectMicroscopy, Videoen
dc.subjectMovementen
dc.subjectNIH 3T3 Cellsen
dc.subjectPinocytosisen
dc.subjectPlasmids/metabolismen
dc.subjectProtein Bindingen
dc.subjectProtein Structure, Tertiaryen
dc.subjectRNA Interferenceen
dc.subjectRecombinant Proteins/chemistryen
dc.subjectTime Factorsen
dc.subjectrab5 GTP-Binding Proteins/*physiologyen
dc.titleThe Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanismsen
heal.abstractThe small GTPase Rab5 is a key regulator of clathrin-mediated endocytosis. On early endosomes, within a spatially restricted domain enriched in phosphatydilinositol-3-phosphate [PI(3)P], Rab5 coordinates a complex network of effectors that functionally cooperate in membrane tethering, fusion, and organelle motility. Here we discovered a novel PI(3)P-binding Rab5 effector, Rabankyrin-5, which localises to early endosomes and stimulates their fusion activity. In addition to early endosomes, however, Rabankyrin-5 localises to large vacuolar structures that correspond to macropinosomes in epithelial cells and fibroblasts. Overexpression of Rabankyrin-5 increases the number of macropinosomes and stimulates fluid-phase uptake, whereas its downregulation inhibits these processes. In polarised epithelial cells, this function is primarily restricted to the apical membrane. Rabankyrin-5 localises to large pinocytic structures underneath the apical surface of kidney proximal tubule cells, and its overexpression in polarised Madin-Darby canine kidney cells stimulates apical but not basolateral, non-clathrin-mediated pinocytosis. In demonstrating a regulatory role in endosome fusion and (macro)pinocytosis, our studies suggest that Rab5 regulates and coordinates different endocytic mechanisms through its effector Rabankyrin-5. Furthermore, its active role in apical pinocytosis in epithelial cells suggests an important function of Rabankyrin-5 in the physiology of polarised cells.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1371/journal.pbio.0020261-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/15328530-
heal.identifier.secondaryhttp://www.plosbiology.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pbio.0020261&representation=PDF-
heal.journalNamePLoS Biolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2004-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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