Effect of ischaemic preconditioning on hepatic oxygenation, microcirculation and function in a rat model of moderate hepatic steatosis
| dc.contributor.author | Koti, R. S. | en |
| dc.contributor.author | Yang, W. | en |
| dc.contributor.author | Glantzounis, G. | en |
| dc.contributor.author | Quaglia, A. | en |
| dc.contributor.author | Davidson, B. R. | en |
| dc.contributor.author | Seifalian, A. M. | en |
| dc.date.accessioned | 2015-11-24T19:22:52Z | |
| dc.date.available | 2015-11-24T19:22:52Z | |
| dc.identifier.issn | 0143-5221 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/22196 | |
| dc.rights | Default Licence | - |
| dc.subject | Adenosine Triphosphate/metabolism | en |
| dc.subject | Animals | en |
| dc.subject | Fatty Liver/metabolism/physiopathology/*surgery | en |
| dc.subject | Liver/*blood supply/metabolism/surgery | en |
| dc.subject | Male | en |
| dc.subject | Microcirculation | en |
| dc.subject | Models, Animal | en |
| dc.subject | Oxidation-Reduction | en |
| dc.subject | Rats | en |
| dc.subject | Rats, Sprague-Dawley | en |
| dc.subject | Reperfusion Injury/metabolism/*physiopathology | en |
| dc.title | Effect of ischaemic preconditioning on hepatic oxygenation, microcirculation and function in a rat model of moderate hepatic steatosis | en |
| heal.abstract | IPC (ischaemic preconditioning) may protect the steatotic liver, which is particularly susceptible to I/R (ischaemia/reperfusion) injury. Hepatic steatosis was induced in Sprague-Dawley rats with a high-cholesterol (2%) diet for 12 weeks after which rats were subjected to I/R (ischaemia/reperfusion; 45 min of lobar ischaemia followed by 2 h of reperfusion). Rats were divided into three study groups (n=6 each) receiving: (i) sham laparotomy alone, (ii) I/R, and (iii) IPC (5 min of ischaemia, followed by 10 min of reperfusion) before I/R. Hepatic extra- and intra-cellular oxygenation and HM (hepatic microcirculation) were measured with near-infrared spectroscopy and laser Doppler flowmetry respectively. Plasma liver enzymes and hepatic tissue ATP were measured as markers of liver injury. Histology showed moderate-grade steatosis in the livers. At the end of 2 h of reperfusion, I/R significantly decreased extra- and intra-cellular oxygenation concomitant with a failure of recovery of HM (21.1+/-14.4% of baseline; P<0.001 compared with sham animals). IPC increased intracellular oxygenation (redox state of the copper centre of cytochrome oxidase; P<0.05 compared with rats receiving I/R alone) and flow in HM (70.9+/-17.1% of baseline; P<0.001 compared with rats receiving I/R alone). Hepatocellular injury was significantly reduced with IPC compared with I/R injury alone (alanine aminotransferase, 474.8+/-122.3 compared with 5436.3+/-984.7 units/l respectively; P<0.01; aspartate aminotransferase, 630.8+/-76.9 compared with 3166.3+/-379.6 units/l respectively; P<0.01]. In conclusion, IPC has a hepatoprotective effect against I/R injury in livers with moderate steatosis. These data may have important clinical implications in liver surgery and transplantation. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1042/CS20040130 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/15341510 | - |
| heal.journalName | Clin Sci (Lond) | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2005 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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