Type III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerization

Απλή σελίδα τεκμηρίου
dc.contributor.authorPozidis, C.en
dc.contributor.authorChalkiadaki, A.en
dc.contributor.authorGomez-Serrano, A.en
dc.contributor.authorStahlberg, H.en
dc.contributor.authorBrown, I.en
dc.contributor.authorTampakaki, A. P.en
dc.contributor.authorLustig, A.en
dc.contributor.authorSianidis, G.en
dc.contributor.authorPolitou, A. S.en
dc.contributor.authorEngel, A.en
dc.contributor.authorPanopoulos, N. J.en
dc.contributor.authorMansfield, J.en
dc.contributor.authorPugsley, A. P.en
dc.contributor.authorKaramanou, S.en
dc.contributor.authorEconomou, A.en
dc.date.accessioned2015-11-24T19:01:06Z
dc.date.available2015-11-24T19:01:06Z
dc.identifier.issn0021-9258-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19643
dc.rightsDefault Licence-
dc.subjectAdenosine Triphosphatases/*chemistry/*physiologyen
dc.subjectAmino Acid Sequenceen
dc.subjectBacterial Proteins/*chemistryen
dc.subjectCell Membrane/enzymology/metabolismen
dc.subjectChromatographyen
dc.subjectCircular Dichroismen
dc.subjectCross-Linking Reagents/pharmacologyen
dc.subjectDetergents/pharmacologyen
dc.subjectDose-Response Relationship, Drugen
dc.subjectEscherichia coli/metabolismen
dc.subjectHydrogen-Ion Concentrationen
dc.subjectHydrolysisen
dc.subjectIonsen
dc.subjectKineticsen
dc.subjectMembrane Transport Proteins/*chemistry/*physiologyen
dc.subjectMicroscopy, Electronen
dc.subjectMolecular Sequence Dataen
dc.subjectPlasmids/metabolismen
dc.subjectProtein Conformationen
dc.subjectProtein Foldingen
dc.subjectProtein Structure, Secondaryen
dc.subjectProtein Structure, Tertiaryen
dc.subjectProtein Transporten
dc.subjectPseudomonas/*enzymologyen
dc.subjectSubcellular Fractionsen
dc.subjectTemperatureen
dc.subjectWater/metabolismen
dc.titleType III protein translocase: HrcN is a peripheral ATPase that is activated by oligomerizationen
heal.abstractType III protein secretion (TTS) is catalyzed by translocases that span both membranes of Gram-negative bacteria. A hydrophilic TTS component homologous to F1/V1-ATPases is ubiquitous and essential for secretion. We show that hrcN encodes the putative TTS ATPase of Pseudomonas syringae pathovar phaseolicola and that HrcN is a peripheral protein that assembles in clusters at the membrane. A decahistidinyl HrcN derivative was overexpressed in Escherichia coli and purified to homogeneity in a folded state. Hydrodynamic analysis, cross-linking, and electron microscopy revealed four distinct HrcN forms: I, 48 kDa (monomer); II, approximately 300 kDa (putative hexamer); III, 575 kDa (dodecamer); and IV, approximately 3.5 MDa. Form III is the predominant form of HrcN at the membrane, and its ATPase activity is dramatically stimulated (>700-fold) over the basal activity of Form I. We propose that TTS ATPases catalyze protein translocation as activated homo-oligomers at the plasma membrane.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1074/jbc.M301903200-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12734178-
heal.identifier.secondaryhttp://www.jbc.org/content/278/28/25816.full.pdf-
heal.journalNameJ Biol Chemen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2003-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Πρωτότυπος φάκελος/πακέτο

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
Pozidis-2003-Type III protein tra.pdf
Μέγεθος:
612.73 KB
Μορφότυπο:
Adobe Portable Document Format
Κατεβάστε

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ