Purification and characterization of a Fusarium oxysporum feruloyl esterase (FoFAE-I) catalysing transesterification of phenolic acid esters
Φόρτωση...
Ημερομηνία
Συγγραφείς
Topakas, E.
Stamatis, H.
Mastihobova, M.
Biely, P.
Kekos, D.
Macris, B. J.
Christakopoulos, P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Enzyme and Microbial Technology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
An extracellular feruloyl esterase (FoFAE-I) from the culture filtrates of Fusarium oxysporum F3 was purified to homogeneity by ion-exchange, hydrophobic interaction and gel filtration chromatographies. The protein corresponded to molecular mass and pI values of 31 kDa and 9.5, respectively. The enzyme was optimally active at pH 7.0 and 55 degreesC. The purified esterase was fully stable at pH 7.0-9.0 and temperature up to 30 degreesC. Determination of k(cat)/K-m revealed that the enzyme hydrolysed methyl p-coumarate (MpCA) 4.5, 9, and 239 times more efficiently than methyl caffeate (MCA), methyl ferulate (MFA) and methyl sinapinate (MSA), respectively. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose but showed preference for the ester at position 2. 4-Nitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside (NPh-5-Fe-Araf) was hydrolysed 100 times more efficiently than 4-nitrophenyl-5-O-trans-feruloyl-alpha-L-arabinofuranoside (NPh-2-Fe-Araf). Ferulic acid (FA) was efficiently released from destarched wheat bran (DSWB) when the esterase was incubated together with xylanase from Sporotrichum thermophile (a maximum of 92% total ferulic acid released after 4 h incubation). FoFAE-I by itself could release FA but at a level almost five-fold lower than that obtained in the presence of xylanase. The potential of FAE-I for the synthesis of various phenolic acid esters was tested using as a reaction system a surfactantless microemulsions formed in ternary mixture consisting of n-hexane, 1-butanol and water. (C) 2003 Elsevier Inc. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
fusarium oxysporum, feruloyl esterase, phenolic acid esters, transesterification, surfactantless microemulsions, sugar-beet pulp, organic-solvent mixtures, aspergillus-niger, cell-walls, wheat bran, detergentless microemulsions, enzymatic-synthesis, cou
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000185366500025
http://ac.els-cdn.com/S0141022903002138/1-s2.0-S0141022903002138-main.pdf?_tid=f184c9ae06623100d554f4f0bc0b0e31&acdnat=1335510221_1384bd6c1df36dce8a8c22dce50c1354
http://ac.els-cdn.com/S0141022903002138/1-s2.0-S0141022903002138-main.pdf?_tid=f184c9ae06623100d554f4f0bc0b0e31&acdnat=1335510221_1384bd6c1df36dce8a8c22dce50c1354
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών