Connective tissue growth factor in pterygium: simultaneous presence with vascular endothelial growth factor - possible contributing factor to conjunctival scarring
Φόρτωση...
Ημερομηνία
Συγγραφείς
van Setten, G.
Aspiotis, M.
Blalock, T. D.
Grotendorst, G.
Schultz, G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Graefes Arch Clin Exp Ophthalmol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
BACKGROUND: Various growth factors have been detected in pterygium and been associated with its vasculogenesis. The basic pathophysiological mechanisms responsible especially for the fibrotic activity in pterygium are, however, not yet known. Connective tissue growth factor (CTGF) has been shown to be substantially involved in various processes of fibrosis. We report on the presence of CTGF in pterygium and its simultaneous presence with vascular endothelial growth factor (VEGF). METHODS: Samples of pterygium were collected during surgery with informed consent of the patients. Specific, non-commercial primary antibodies against CTGF were used to detect CTGF using immunohistochemistry. Specificity of antibodies was confirmed with Western-blot analysis. The same specimens were stained with commercial antibodies for VEGF. Additionally RT-PCR analysis was performed from pterygium samples. RESULTS: CTGF was detected in the epithelium of all samples as well as in some stromal keratocytes. The RT-PCR confirmed the identity of CTGF in these samples. The staining pattern differed slightly from that of VEGF, which was detected in all samples. The control sections were negative. CONCLUSION: CTGF is present in the epithelium of a majority of pterygia and probably contributes to fibrosis. Simultaneous presence with VEGF suggests growth factor interaction and possible involvement in apoptotic dysregulation.
Περιγραφή
Λέξεις-κλειδιά
Blotting, Western, Cicatrix/metabolism, Conjunctival Diseases/*metabolism, Connective Tissue Growth Factor, Endothelial Growth Factors/genetics/*metabolism, Epithelium/metabolism, Humans, Immediate-Early Proteins/genetics/*metabolism, Immunoenzyme Techniques, Intercellular Signaling Peptides and Proteins/genetics/*metabolism, Lymphokines/genetics/*metabolism, Pterygium/*metabolism, RNA, Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/12605268
http://www.springerlink.com/content/g82p1aq18dp2wy8t/fulltext.pdf
http://www.springerlink.com/content/g82p1aq18dp2wy8t/fulltext.pdf
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής