Properties of catechol 1,2-dioxygenase from Pseudomonas putida immobilized in calcium alginate hydrogels
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kalogeris, E.
Sanakis, Y.
Mamma, D.
Christakopoulos, P.
Kekos, D.
Stamatis, H.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Enzyme and Microbial Technology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Catechol 1,2-dioxygenase from Pseudomonas putida was isolated and immobilized in calcium alginate hydrogels. The gel matrix could effectively entrap the enzyme, with high retention of activity. Following immobilization, catechol 1,2-dioxygenase exhibited improved storage stability and activity in the presence of organic solvents, and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficiency after successive operational cycles. The hypothesis that enhancement of enzyme stability after immobilization is related to the stabilization of its multimeric structure has been investigated. Electron paramagnetic resonance (EPR) spectroscopy indicates that the environment of the non-heme iron center was not affected during the immobilization process and the ability for the substrate (catechol) binding at the metal center was retained. Catalytic constants for free and immobilized enzyme were practically equivalent. The influence of internal and external mass-transfer limitations on the initial reaction rates of dioxygenase-catalyzed oxidation reactions has been investigated. (c) 2006 Elsevier Inc. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
catechol 1,2-dioxygenase, immobilization, alginate, hydrogel, epr spectroscopy, mass transfer, pseudomonas putida, acinetobacter-radioresistens s13, multimeric enzymes, ampicillin synthesis, organic-solvents, meta-pathway, stabilization, stability, diox
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000239694500022
http://ac.els-cdn.com/S0141022906001232/1-s2.0-S0141022906001232-main.pdf?_tid=1ca75659a27f202d59c7388f2acab842&acdnat=1335510864_a7e94fe8a2756363431557c35b2f5d0c
http://ac.els-cdn.com/S0141022906001232/1-s2.0-S0141022906001232-main.pdf?_tid=1ca75659a27f202d59c7388f2acab842&acdnat=1335510864_a7e94fe8a2756363431557c35b2f5d0c
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών