Distribution of Paf-Acetylhydrolase Activity in Human Plasma Low-Density-Lipoprotein Subfractions
Φόρτωση...
Ημερομηνία
Συγγραφείς
Karabina, S. A. P.
Liapikos, T. A.
Grekas, G.
Goudevenos, J.
Tselepis, A. D.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Biochimica Et Biophysica Acta-Lipids and Lipid Metabolism
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The distribution of PAF-acetylhydrolase (PAF-AH) activity in 3 LDL subfractions prepared by density gradient ultracentrifugation as well as the rate of phosphatidylcholine (PC) hydrolysis during oxidation was studied. PAF-AH activity, measured before oxidation, was much higher in LDL(3) subfraction (28.4 +/- 8.6 nmol/mg per min) comparing to LDL(2) (14.1 +/- 5.8 nmol/mg per min), and to LDL(1), 8.7 +/- 3.7 nmol/mg per min. During oxidation, the enzyme activity was continuously decreased and this phenomenon was more pronounced in LDL(1). PC hydrolysis was studied measuring the lyso-PC production expressed as lyso-PC/Sph molar ratio. Before oxidation, the lyso-PC/Sph molar ratio, did not differ significantly among the LDL subfractions, whereas, 4 h after the onset of oxidation, it was significantly higher in LDL(2) and LDL(3) subfractions (0.42 +/- 0.12 and 0.45 +/- 0.10, respectively), comparing to LDL(1) (0.29 +/- 0.06). Our results show that the distribution of PAF-AH activity in LDL subfractions is heterogeneous (mainly distributed in LDL(2) and LDL(3) subfractions) and it is positively correlated with higher lyso-PC production in those subfractions during oxidation. The contribution of this phenomenon to the enhanced susceptibility to oxidation as well as to the higher atherogenicity of the dense LDL subfractions is under investigation.
Περιγραφή
Λέξεις-κλειδιά
paf-acetylhydrolase, ldl, ldl subfraction, oxidized ldl, lipid peroxidation, platelet-activating-factor, lipid-peroxidation, invitro oxidation, degradation, phosphatidylcholine, phospholipids, atherogenesis, cells, risk, ldl
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://A1994NU79700006
http://ac.els-cdn.com/0005276094902194/1-s2.0-0005276094902194-main.pdf?_tid=1a3cf3e82d7f0a2bb5b92d49b01c5679&acdnat=1333111342_c5242d07b43c6e38f378a917a197728c
http://ac.els-cdn.com/0005276094902194/1-s2.0-0005276094902194-main.pdf?_tid=1a3cf3e82d7f0a2bb5b92d49b01c5679&acdnat=1333111342_c5242d07b43c6e38f378a917a197728c
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας