Human platelets secrete the plasma type of platelet-activating factor acetylhydrolase primarily associated with microparticles
| dc.contributor.author | Mitsios, J. V. | en |
| dc.contributor.author | Vini, M. P. | en |
| dc.contributor.author | Stengel, D. | en |
| dc.contributor.author | Ninio, E. | en |
| dc.contributor.author | Tselepis, A. D. | en |
| dc.date.accessioned | 2015-11-24T16:39:09Z | |
| dc.date.available | 2015-11-24T16:39:09Z | |
| dc.identifier.issn | 1079-5642 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/8125 | |
| dc.rights | Default Licence | - |
| dc.subject | atherosclerosis | en |
| dc.subject | inflammation | en |
| dc.subject | paf | en |
| dc.subject | paf-acetyhydrolase | en |
| dc.subject | pmps | en |
| dc.subject | lipoprotein-associated phospholipase-a2 | en |
| dc.subject | paf-degrading acetylhydrolase | en |
| dc.subject | acute coronary syndromes | en |
| dc.subject | membrane microparticles | en |
| dc.subject | bioactive lipids | en |
| dc.subject | shear-stress | en |
| dc.subject | procoagulant | en |
| dc.subject | enzyme | en |
| dc.subject | purification | en |
| dc.subject | inhibitor | en |
| dc.title | Human platelets secrete the plasma type of platelet-activating factor acetylhydrolase primarily associated with microparticles | en |
| heal.abstract | Objective-Platelet-activating factor acetylhydrolase (PAF-AH) expresses a Ca2+-independent phospholipase A, activity and hydrolyzes platelet-activating factor as well as oxidized phospholipids. Two major types of PAF-AH have been described: the plasma type, which is associated with lipoproteins, and the intracellular type II PAF-AH. Methods and Results-We investigated the type(s) of PAF-AH expressed in human platelets as well as the mechanism and the enzyme type secreted from platelets during activation. The majority of the enzyme activity (75.1 +/- 14.3% of total) is found in the cytosol, whereas 24.9 +/- 7.3% is associated with the membranes. Immunofluorescence microscopy studies and Western blotting analysis showed that platelets contain the plasma type as well as the intracellular type II PAF-AH. Furthermore, platelets contain high levels of the mRNA of plasma PAF-AH, whereas only a small quantity of the type II PAF-AH mRNA was detected. On activation, platelets secrete the plasma type of PAF-AH mainly associated with platelet-derived microparticles (PMPs). The enzyme activity was also detected on circulating PMPs in plasma from normolipidemic healthy subjects. Conclusion-This is the first indication that in addition to lipoproteins, PAF-AH in human plasma is carried by PMPs, suggesting that the PMP-associated PAF-AH may play a role in the dissemination of biological activities mediated by these particles. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | Doi 10.1161/01.Atv.0000228821.79588.Ef | - |
| heal.identifier.secondary | <Go to ISI>://000239391400034 | - |
| heal.identifier.secondary | http://atvb.ahajournals.org/content/26/8/1907.full.pdf | - |
| heal.journalName | Arteriosclerosis Thrombosis and Vascular Biology | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2006 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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