Phenobarbital enhances the aldehyde dehydrogenase activity of rat hepatocytes in vitro and in vivo
Φόρτωση...
Ημερομηνία
Συγγραφείς
Marselos, M.
Michalopoulos, G.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Acta Pharmacol Toxicol (Copenh)
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Aldehyde dehydrogenase (ALDH) was measured in primary cultures of hepatocytes obtained with collagenase perfusion from livers of Long-Evans rats. After seven days in culture, basal ALDH activity, protein content and DNA content are significantly decreased. Exposure of the cultures to phenobarbital (PB, 3 mM in the media) does not prevent the decrease of DNA content, although it keeps protein at relatively higher levels. The activity of ALDH is not only preserved, but also significantly enhanced, when propionaldehyde, phenylacetaldehyde, benzaldehyde and D-glucuronolactone are used as substrates and NAD as the coenzyme. A relative increase of activity is also noted when ALDH is measured with benzaldehyde and NADP. Treatment of Long-Evans animals with PB (1 mg/ml, in drinking water for 2 weeks) leads to similar relative increases of the ALDH activity. In absolute values, however, enzyme activities found after in vivo treatment with PB are higher, compared to those obtained after in vitro exposure. These results show that ALDH activity can be greatly enhanced by PB in primary hepatocyte cultures, free from any indirect endogenous influences.
Περιγραφή
Λέξεις-κλειδιά
Aldehyde Dehydrogenase/*metabolism, Animals, DNA/metabolism, Enzyme Activation/drug effects, Isoenzymes/*metabolism, Liver/*enzymology, Male, Phenobarbital/*pharmacology, Rats, Substrate Specificity
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/3811968
http://onlinelibrary.wiley.com/store/10.1111/j.1600-0773.1986.tb00191.x/asset/j.1600-0773.1986.tb00191.x.pdf?v=1&t=h095f11l&s=c47c7cfff02c7ca28d853e3d333e9c203b272d27
http://onlinelibrary.wiley.com/store/10.1111/j.1600-0773.1986.tb00191.x/asset/j.1600-0773.1986.tb00191.x.pdf?v=1&t=h095f11l&s=c47c7cfff02c7ca28d853e3d333e9c203b272d27
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής