Modulation of cystatin C expression impairs the invasive and tumorigenic potential of human glioblastoma cells

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dc.contributor.authorKonduri, S. D.en
dc.contributor.authorYanamandra, N.en
dc.contributor.authorSiddique, K.en
dc.contributor.authorJoseph, A.en
dc.contributor.authorDinh, D. H.en
dc.contributor.authorOlivero, W. C.en
dc.contributor.authorGujrati, M.en
dc.contributor.authorKouraklis, G.en
dc.contributor.authorSwaroop, A.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T19:04:09Z
dc.date.available2015-11-24T19:04:09Z
dc.identifier.issn0950-9232-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19983
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectBrain Neoplasms/genetics/*pathologyen
dc.subjectCoculture Techniquesen
dc.subjectCystatin Cen
dc.subjectCystatins/*geneticsen
dc.subjectGene Expression Regulation, Neoplastic/geneticsen
dc.subjectGlioblastoma/genetics/*pathologyen
dc.subjectHumansen
dc.subjectMiceen
dc.subjectMice, Nudeen
dc.subjectNeoplasm Transplantationen
dc.subjectRNA, Messenger/geneticsen
dc.subjectRatsen
dc.subjectTransfectionen
dc.subjectTumor Cells, Cultureden
dc.titleModulation of cystatin C expression impairs the invasive and tumorigenic potential of human glioblastoma cellsen
heal.abstractIncreases in the abundance of cathepsin B transcript and protein with increased tumor grade and changes in subcellular localization and activity of this enzyme. We observed progressive reductions in levels of the protease inhibitor cystatin C, an inhibitor of cathepsin B with corresponding increases in the malignancy of glioma cell lines, implying an inverse correlation between cystatin C and tumor grade. To investigate the role of cystatin C in the invasion of brain tumor cells, we stably transfected SNB19 glioblastoma cells with either a 0.4-kb cDNA construct of human cystatin C in the sense orientation or an empty vector. Clones expressing sense-cystatin C cDNA had higher cystatin C mRNA and protein levels than did control cells. Sense-transfected cells were also markedly less invasive than control cells in a Matrigel invasion assay and in a coculture assay of SNB19 spheroids and fetal rat brain aggregates. Finally, the sense-transfected cells did not form tumors in nude mice upon intracerebral injection. These results strongly implicate cystatin C in the invasiveness of human glioblastoma cells and suggest that sense transcripts of cystatin C may prove useful in cancer therapy.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1038/sj.onc.1205949-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12483523-
heal.identifier.secondaryhttp://www.nature.com/onc/journal/v21/n57/pdf/1205949a.pdf-
heal.journalNameOncogeneen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2002-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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