Comparative proteomic analysis of alcoholic fermentation employing a new environmental strain of Saccharomyces cerevisiae
Φόρτωση...
Ημερομηνία
Συγγραφείς
Parapouli, M.
Fragkos-Livanios, L.
Samiotaki, M.
Koukkou, A. I.
Perisynakis, A.
Hatziloukas, E.
Panayotou, G.
Drainas, C.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Process Biochemistry
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The aim of this study was to investigate the biochemical pathways induced during must fermentation employing the Saccharomyces cerevisiae, strain Z622, isolated from the traditional wine area of Zitsa (Epirus, Greece). Proteomic analysis (two-dimensional gel electrophoresis followed by nano-LC ESI-MS/MS) was used to assess the protein profile response of strain Z622 cultured in Debina grape must, yeast extract-malt extract (YM) or minimal medium (MM) under fermentation conditions, at the beginning and at the end of fermentation. The proteomic results and their analysis with Gene Ontology (GO) terms revealed that the intracellular proteins induced in yeast cells grown exclusively in Debina grape must during the early stage of fermentation were mainly involved in amino acid metabolism, glycolysis and sterol formation. These three pathways reflect the adaptation of the cell to the fermentative metabolism. During the late stage, several enzymes were induced due to starvation conditions or to participation in defence mechanisms against oxidative stress and protein degradation. Several of the identified enzymes have not previously been reported to be induced during wine fermentation and thus the results presented here can contribute to a better understanding of how S. cerevisiae cells adapt to wine fermentation. (C) 2010 Elsevier Ltd. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
saccharomyces cerevisiae, comparative proteomics, wine fermentation, wine quality, wine yeast, debina must, wine yeast, oxidative stress, ethanol tolerance, expression, adaptation, metabolism, gene, carboxypeptidase, biosynthesis, responses
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000279206400011
http://ac.els-cdn.com/S1359511310001054/1-s2.0-S1359511310001054-main.pdf?_tid=2397df26-357f-11e3-a8e6-00000aab0f6b&acdnat=1381830772_782364f141a8cb95ea1682c83e77be96
http://ac.els-cdn.com/S1359511310001054/1-s2.0-S1359511310001054-main.pdf?_tid=2397df26-357f-11e3-a8e6-00000aab0f6b&acdnat=1381830772_782364f141a8cb95ea1682c83e77be96
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας