The detection of glucocorticoid receptors in breast cancer by immunocytochemical and biochemical methods
Φόρτωση...
Ημερομηνία
Συγγραφείς
Ioannidis, C.
Papamichail, M.
Agnanti, N.
Garas, J.
Tsawdaroglou, N.
Sekeris, C. E.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Int J Cancer
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Glucocorticoid receptors have been detected in 90 human breast tumors and tumor-like conditions by the immunoperoxidase method using a specific antibody against the glucocorticoid receptor isolated from rat thymocytes. In some of the specimens the [3H]-dexamethasone binding assay was also performed and the results obtained were compared with those of the immunoperoxidase method. When the biochemical method was used, no strict correlation between the degree of binding of [3H]-dexamethasone and malignancy on the basis of histological findings could be demonstrated. In contrast, the immunoperoxidase method was in full agreement with the histological type of the tumor. Thus, nearly all malignant breast tumors (carcinomas) were positive by the immunoperoxidase method to a vary degree. Most of the examined benign tumor-like conditions (fibrocystic disease) were found to be negative. Intermediate situations, such as atypical duct of lobular hyperplasia, papillomatosis etc, were mostly positive. These findings suggest that ther immunoperoxidase method, part from its usefulness for the detection of glucocorticoid receptors in breast tissue, may be used as an early biological marker to detect early conversion of normal to hyperplastic tissue and/or malignancy of the mammary gland.
Περιγραφή
Λέξεις-κλειδιά
Breast Neoplasms/*metabolism/pathology, Dexamethasone/metabolism, Female, Fibrocystic Breast Disease/metabolism/pathology, Humans, Immunoenzyme Techniques, Receptors, Glucocorticoid/*analysis, Receptors, Steroid/*analysis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/7037657
http://onlinelibrary.wiley.com/store/10.1002/ijc.2910290206/asset/2910290206_ftp.pdf?v=1&t=h1sz18ji&s=605ffe4282e6b460475b2b979179c966fc431f3a
http://onlinelibrary.wiley.com/store/10.1002/ijc.2910290206/asset/2910290206_ftp.pdf?v=1&t=h1sz18ji&s=605ffe4282e6b460475b2b979179c966fc431f3a
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής