Altered actin cytoskeleton and inhibition of matrix metalloproteinase expression by vanadate and phenylarsine oxide, inhibitors of phosphotyrosine phosphatases: modulation of migration and invasion of human malignant glioma cells

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dc.contributor.authorChintala, S. K.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorMohan, P. M.en
dc.contributor.authorMohanam, S.en
dc.contributor.authorSawaya, R.en
dc.contributor.authorGokslan, Z.en
dc.contributor.authorYung, W. K.en
dc.contributor.authorSteck, P.en
dc.contributor.authorUhm, J. H.en
dc.contributor.authorAggarwal, B. B.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T19:36:53Z
dc.date.available2015-11-24T19:36:53Z
dc.identifier.issn0899-1987-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23958
dc.rightsDefault Licence-
dc.subjectActins/*drug effectsen
dc.subjectArsenicals/*pharmacologyen
dc.subjectCell Adhesion Molecules/geneticsen
dc.subjectCell Movement/*drug effectsen
dc.subjectCytoskeleton/*drug effectsen
dc.subjectEnzyme Inhibitors/pharmacologyen
dc.subjectFocal Adhesion Kinase 1en
dc.subjectFocal Adhesion Protein-Tyrosine Kinasesen
dc.subjectGene Expression Regulation, Neoplastic/*drug effectsen
dc.subjectGliomaen
dc.subjectHumansen
dc.subjectMatrix Metalloproteinases/*geneticsen
dc.subjectNeoplasm Invasiveness/*prevention & controlen
dc.subjectPhosphoproteins/metabolismen
dc.subjectPhosphotyrosine/analysisen
dc.subjectProtein Tyrosine Phosphatases/antagonists & inhibitors/*metabolismen
dc.subjectProtein-Tyrosine Kinases/geneticsen
dc.subjectTetradecanoylphorbol Acetate/pharmacologyen
dc.subjectTumor Cells, Cultureden
dc.subjectVanadates/*pharmacologyen
dc.titleAltered actin cytoskeleton and inhibition of matrix metalloproteinase expression by vanadate and phenylarsine oxide, inhibitors of phosphotyrosine phosphatases: modulation of migration and invasion of human malignant glioma cellsen
heal.abstractCell-matrix interactions exert a profound influence on cell function and behavior. Our earlier observations suggested that disruption of the actin cytoskeleton results in the inhibition of phorbol ester-induced matrix metalloproteinase (MMP)-9 expression. In this study, to understand the role of protein tyrosine phosphatases in matrix metalloproteinase-9 expression, we treated glioblastoma cells with vanadate and phenylarsine oxide (PAO), which are inhibitors of protein tyrosine phosphatases. Vanadate and PAO inhibited expression of phorbol ester-induced MMP-9 as well as constitutive expression of matrix metalloproteinase-2 in a dose- and time-dependent fashion. An assay of the activity of phosphotyrosine phosphatase (PTPase) indicated that vanadate-treated cells had reduced PTPase activity compared with that of untreated controls. Vanadate and PAO also inhibited actin polymerization, cell spreading, migration, and invasion of glioma cells. Furthermore, elevated levels of protein tyrosine phosphorylation were observed in vanadate- and PAO-treated cells in both a concentration- and time-dependent fashion and were seen to have an inverse correlation with focal adhesion kinase protein expression. These results suggest that vanadate and PAO inhibited migration and invasion of glioma cells by their effect on the cytoskeleton and inhibition of MMP expression.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10569804-
heal.journalNameMol Carcinogen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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