Capillary gas chromatographic method for the analysis of lignans in human urine
| dc.contributor.author | Fotsis, T. | en |
| dc.contributor.author | Heikkinen, R. | en |
| dc.contributor.author | Adlercreutz, H. | en |
| dc.contributor.author | Axelson, M. | en |
| dc.contributor.author | Setchell, K. D. | en |
| dc.date.accessioned | 2015-11-24T19:31:40Z | |
| dc.date.available | 2015-11-24T19:31:40Z | |
| dc.identifier.issn | 0009-8981 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23305 | |
| dc.rights | Default Licence | - |
| dc.subject | 4-Butyrolactone/analogs & derivatives/urine | en |
| dc.subject | Adult | en |
| dc.subject | Butylene Glycols/urine | en |
| dc.subject | Chromatography, Gas/*methods | en |
| dc.subject | Chromatography, Ion Exchange | en |
| dc.subject | Female | en |
| dc.subject | Gas Chromatography-Mass Spectrometry | en |
| dc.subject | Glucuronates/urine | en |
| dc.subject | Humans | en |
| dc.subject | Lignans | en |
| dc.subject | Male | en |
| dc.subject | Middle Aged | en |
| dc.subject | Plant Extracts/*urine | en |
| dc.title | Capillary gas chromatographic method for the analysis of lignans in human urine | en |
| heal.abstract | We describe a capillary column gas chromatographic (GC) method for the analysis of lignans in urine. Lignans are excreted as mono-glucuronides which are first extracted on a small reversed-phase cartridge of octadecylsilane bonded silica (Sep-Pak C18) and thereafter isolated by anion exchange chromatography on DEAE-Sephadex A-25, prepared in the acetate form. Lignan mono-glucuronides are enzymatically hydrolysed and re-extracted on a Sep-Pak C18 cartridge. Quantification is carried out by capillary column GC of the trimethylsilyl ether derivatives. The specificity of the method was checked by GC/MS and the intra-assay coefficient of variation (CV) for the two lignans, enterolactone (trans-2,3-bis(3-hydroxybenzyl)butyrolactone) and enterodiol (2.3-bis(3-hydroxybenzyl)butane-1,4-diol) varied between 5 and 8%. Some values for the excretion of these lignans by normal men and women are presented. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/6286168 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/0009898182902455/1-s2.0-0009898182902455-main.pdf?_tid=ac7a94436721f69baec51c0ad32fdd9b&acdnat=1332751328_27f5d674ca9b024bd38f19efb870fa07 | - |
| heal.journalName | Clin Chim Acta | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1982 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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