Clone-specific high-frequency retrotransposition of a recombinant virus containing a VL30 promoter in SV40-transformed NIH3T3 cells

Απλή σελίδα τεκμηρίου
dc.contributor.authorTzavaras, T.en
dc.contributor.authorKalogera, C.en
dc.contributor.authorEftaxia, S.en
dc.contributor.authorSaragosti, S.en
dc.contributor.authorPagoulatos, G. N.en
dc.date.accessioned2015-11-24T19:30:12Z
dc.date.available2015-11-24T19:30:12Z
dc.identifier.issn0006-3002-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/23039
dc.rightsDefault Licence-
dc.subject3T3 Cellsen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectCell Transformation, Viral/*geneticsen
dc.subjectDNA Probesen
dc.subjectGenes, Reporteren
dc.subjectMiceen
dc.subjectPlasmidsen
dc.subject*Promoter Regions, Geneticen
dc.subjectRecombination, Geneticen
dc.subject*Repetitive Sequences, Nucleic Aciden
dc.subjectRestriction Mappingen
dc.subject*Retroelementsen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectSimian virus 40/*geneticsen
dc.subjectTransfectionen
dc.titleClone-specific high-frequency retrotransposition of a recombinant virus containing a VL30 promoter in SV40-transformed NIH3T3 cellsen
heal.abstractA recombinant virus, containing the promoter of a VL30 LTR and tagged with the neomycin gene as a selection and indicator marker, was constructed to investigate transposition events in NIH3T3 cells after SV40 transformation. This retroviral construct was transfected into psi/CRE packaging cells, and pseudovirions were used to infect NIH3T3 cells. Clones resistant to G418 bearing single-copy integrations of the recombinant virus were isolated and transformed by SV40 virus. Transpositions were detected through RFLPs with a neomycin probe and 'retrotransposition' was further confirmed by inverse PCR and DNA sequencing of transposed and parental copies. We found that: (1) retrotransposition of this recombinant virus occurred with a high frequency in a parental clone transformed with SV40 virus suggesting that the frequency of retrotransposition depended on the initial site of provirus integration; (2) the transposition frequency was independent of the transcription level of the recombinant construct; and (3) analysis of transposition-positive transformants showed that the high transposition frequency appeared to be associated with the induction of endogenous reverse transcriptases.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9804952-
heal.journalNameBiochim Biophys Actaen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1998-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ