Specific inhibition of simian virus 40 protein synthesis by heat and arsenite treatment
Φόρτωση...
Ημερομηνία
Συγγραφείς
Angelidis, C. E.
Lazaridis, I.
Pagoulatos, G. N.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Eur J Biochem
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The effects of heat treatment of CV1 cells infected with simian virus 40 (SV40) on viral and cellular protein synthesis were investigated by one-dimensional and two-dimensional polyacrylamide gel electrophoresis. A 12-h heat treatment during the late phase of the viral life-cycle inhibits VP1 synthesis. No inhibition of normal cellular proteins is apparent, but heat-shock proteins are strongly induced and accumulate in the cells. Inhibition of VP1 synthesis in infected cells is demonstrated to occur also after arsenite treatment, another agent known to induce heat-shock proteins. Northern blot analysis of cytoplasmic RNA demonstrated a decrease in the abundance of late SV40 mRNAs thus showing that the inhibition occurs at the transcriptional or immediately post-transcriptional level. Cumulative labeling with [3H]thymidine of viral DNA showed that the decrease in the abundance of late mRNAs is not due to a blocking of viral DNA synthesis. Immunofluorescence microscopy and immunoprecipitation analysis show that heat and arsenite treatments also affect the synthesis of T antigen. These results suggest that heat-shock proteins may play a role in the inhibition of SV40 virus gene functions.
Περιγραφή
Λέξεις-κλειδιά
Antigens, Viral, Tumor/biosynthesis, Arsenic/*pharmacology, *Arsenites, DNA Replication, DNA, Viral/analysis, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Heat-Shock Proteins/biosynthesis, *Hot Temperature, Nucleic Acid Hybridization, RNA/analysis, RNA, Messenger/analysis, Simian virus 40/drug effects/genetics/*metabolism, Viral Proteins/*biosynthesis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/2450024
http://onlinelibrary.wiley.com/store/10.1111/j.1432-1033.1988.tb13851.x/asset/j.1432-1033.1988.tb13851.x.pdf?v=1&t=h2bp5dd0&s=73136674eda3f0cf592a545e03478cf6520c3257
http://onlinelibrary.wiley.com/store/10.1111/j.1432-1033.1988.tb13851.x/asset/j.1432-1033.1988.tb13851.x.pdf?v=1&t=h2bp5dd0&s=73136674eda3f0cf592a545e03478cf6520c3257
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής