Release of cell-free ice nuclei from Halomonas elongata expressing the ice nucleation gene inaZ of Pseudomonas syringae
| dc.contributor.author | Tegos, G. | en |
| dc.contributor.author | Vargas, C. | en |
| dc.contributor.author | Perysinakis, A. | en |
| dc.contributor.author | Koukkou, A. I. | en |
| dc.contributor.author | Christogianni, A. | en |
| dc.contributor.author | Nieto, J. J. | en |
| dc.contributor.author | Ventosa, A. | en |
| dc.contributor.author | Drainas, C. | en |
| dc.date.accessioned | 2015-11-24T16:46:22Z | |
| dc.date.available | 2015-11-24T16:46:22Z | |
| dc.identifier.issn | 1364-5072 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/9063 | |
| dc.rights | Default Licence | - |
| dc.subject | moderately halophilic eubacteria | en |
| dc.subject | escherichia-coli | en |
| dc.subject | biotechnological applications | en |
| dc.subject | erwinia-herbicola | en |
| dc.subject | reporter gene | en |
| dc.subject | protein | en |
| dc.subject | identification | en |
| dc.subject | principles | en |
| dc.subject | repetition | en |
| dc.subject | plasmid | en |
| dc.title | Release of cell-free ice nuclei from Halomonas elongata expressing the ice nucleation gene inaZ of Pseudomonas syringae | en |
| heal.abstract | Release of ice nuclei in the growth medium of recombinant Halomonas elongata cells expressing the inaZ gene of Pseudomonas syringae was studied in an attempt to produce cell-free active ice nuclei for biotechnological applications. Cell-free ice nuclei were not retained by cellulose acetate filters of 0.2 mum pore size. Highest activity of cell-free ice nuclei was obtained when cells were grown in low salinity (0.5-5% NaCl, w/v). Freezing temperature threshold, estimated to be below - 7 degreesC indicating class C nuclei, was not affected by medium salinity. Their density, as estimated by Percoll density centrifugation, was 1.018 +/- 0.002 g ml(-1) and they were found to be free of lipids. Ice nuclei are released in the growth medium of recombinant H. elongata cells probably because of inefficient anchoring of the ice-nucleation protein aggregates in the outer membrane. The ice(+) recombinant H. elongata cells could be useful for future use as a source of active cell-free ice nucleation protein. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | <Go to ISI>://000165499800008 | - |
| heal.identifier.secondary | http://onlinelibrary.wiley.com/store/10.1046/j.1365-2672.2000.01180.x/asset/j.1365-2672.2000.01180.x.pdf?v=1&t=h0dxi3tf&s=4e8642c12643e5373c988c1965e0d87d0ca479a6 | - |
| heal.journalName | J Appl Microbiol | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2000 | - |
| heal.publisher | Wiley-Blackwell | en |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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