Contribution of chemical changes in membrane lipids to the osmoadaptation of the halophilic bacterium Chromohalobacter salexigens

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dc.contributor.authorVargas, C.en
dc.contributor.authorKallimanis, A.en
dc.contributor.authorKoukkou, A. I.en
dc.contributor.authorCalderon, M. I.en
dc.contributor.authorCanovas, D.en
dc.contributor.authorIglesias-Guerra, F.en
dc.contributor.authorDrainas, C.en
dc.contributor.authorVentosa, A.en
dc.contributor.authorNieto, J. J.en
dc.date.accessioned2015-11-24T16:54:49Z
dc.date.available2015-11-24T16:54:49Z
dc.identifier.issn0723-2020-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/10203
dc.rightsDefault Licence-
dc.subjectosmoadaptationen
dc.subjectchromohalobacter salexigensen
dc.subjectmembrane lipidsen
dc.subjectectoineen
dc.subjectfatty-acid-compositionen
dc.subjectcompatible solute ectoineen
dc.subjecthalomonas-elongataen
dc.subjectsalt concentrationen
dc.subjectescherichia-colien
dc.subjectpseudomonas-halosaccharolyticaen
dc.subjectgrowth temperatureen
dc.subjectbiosynthesisen
dc.subjectstressen
dc.subjecttoleranceen
dc.titleContribution of chemical changes in membrane lipids to the osmoadaptation of the halophilic bacterium Chromohalobacter salexigensen
heal.abstractThe long-term response of the broad-salt growing halophile Chromohalobacter salexigens DSM 3043(T) to salt stress has been investigated with respect to adaptive changes in membrane lipid composition. This study included the wildtype and three salt-sensitive, ectoine-deficient strains: CHR62 (ectA::Tn1732, unable to grow above 0.75M NaCl), CHR63 (eetC::Tn1732, unable to grow above 1.5 M NaCl), and CHR64, which was able to grow in minimal medium M63 up to 2.5 M NaCl, but its growth was slower than the wild-type strain at salinities above 1.5 M NaCl. This mutant accumulated ectoine and hydroxyectoine as major compatible solutes, but also the ectoine precursor, N-gamma-acetyldiaminobutyric acid, and was found to be affected in the ectoine synthase gene ectC. The main phospholipids of the wild-type strain were phosphatidylethanolamine, phosphatidylglycerol (PG), and cardiolipin (CL). Major fatty acids were detected as 16:0, 18:1, and 16:1, including significant amounts of cyc-19:0, and cyc-17:0. CL and cyclopropane fatty acids (CFA) levels were elevated when the wild-type strain was grown at high salinity (2.5 M NaCl). Membranes of the most salt-sensitive trains CHR62 and CHR63, but not of the less salt-sensitive strain CHR64, contained lower levels of CL. The proportion of cyc-19:0 in CHR64 was three-fold (at 2.0 M NaCl) and 2.5-fold (at 2.5 M NaCl) lower than that of the wild type, suggesting that this mutant has a limited capacity to incorporate CFA into phospholipids at high salt. The addition of 1 mM ectoine to cultures of the wild-type strain increased the ratio PG/ CL from 1.8 to 3.3 at 0.75 M NaCl, and from 1 to 6.5 at 2.5 M NaCl, and led to a slight decrease in CFA content. Addition of 1 mM ectoine to the mutants restored the steady-state levels of CL and CFA found in the wild-type strain supplemented with ectoine. These findings suggest that exogenous ectoine might attenuate the osmostress response involving changes in membrane lipids. (c) 2005 Elsevier GmbH. All rights reserved.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1016/j.syapm.2005.03.014-
heal.identifier.secondary<Go to ISI>://000231799700001-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0723202005000834/1-s2.0-S0723202005000834-main.pdf?_tid=3b89d292-357f-11e3-96b3-00000aab0f6c&acdnat=1381830812_fb139543cba4e6e8a3bc9231f9ae7822-
heal.journalNameSystematic and Applied Microbiologyen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2005-
heal.publisherElsevieren
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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