Soluble cAMP-independent protein kinase from human spleen
| dc.contributor.author | Gounaris, A. | en |
| dc.contributor.author | Trangas, T. T. | en |
| dc.contributor.author | Tsiapalis, C. M. | en |
| dc.date.accessioned | 2015-11-24T16:31:42Z | |
| dc.date.available | 2015-11-24T16:31:42Z | |
| dc.identifier.issn | 0003-9861 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7473 | |
| dc.rights | Default Licence | - |
| dc.subject | Adenosine Triphosphate/metabolism | en |
| dc.subject | Chromatography, Gel | en |
| dc.subject | Cyclic AMP/*metabolism | en |
| dc.subject | Guanosine Triphosphate/metabolism | en |
| dc.subject | Humans | en |
| dc.subject | Isoelectric Point | en |
| dc.subject | Kinetics | en |
| dc.subject | Molecular Weight | en |
| dc.subject | Protein Kinases/*metabolism | en |
| dc.subject | Spleen/*enzymology | en |
| dc.subject | Substrate Specificity | en |
| dc.title | Soluble cAMP-independent protein kinase from human spleen | en |
| heal.abstract | A protein kinase (EC 2.7.1.37) was purified 2000-fold, from the soluble protein fraction of human spleen cells, using ion-exchange chromatography, ammonium sulfate fractionation, and gel filtration. This rapid procedure yielded 30% of the initial activity and an enzyme preparation with specific activity of 62 nmol min-1 mg-1 of protein. On the basis of disc gel electrophoresis in sodium dodecyl sulfate acrylamide gels and isoelectric focusing the enzyme preparation appears homogeneous and to consist of one polypeptide with a molecular weight of 43,000 and having a pI of 7.1. The purified enzyme activity is cyclic AMP and cGMP independent phosphorylates both alpha-casein and phosvitin, and uses Mg2+ ATP and Mg2+ GTP as phosphate donors, exhibiting an apparent Km of 2.0 and 6.6 X 10(-5)m, respectively. Furthermore, the enzyme activity is strongly inhibited by heparin (K50 = 0.1 micrograms/ml). These catalytic properties are characteristic of the enzyme casein kinase II, as described in several eukaryotic cells. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/2827577 | - |
| heal.journalName | Arch Biochem Biophys | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1987 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
Αρχεία
Φάκελος/Πακέτο αδειών
1 - 1 of 1
Φόρτωση...
- Ονομα:
- license.txt
- Μέγεθος:
- 1.74 KB
- Μορφότυπο:
- Item-specific license agreed upon to submission
- Περιγραφή: