Adenovirus-mediated p16/CDKN2 gene transfer suppresses glioma invasion in vitro

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dc.contributor.authorChintala, S. K.en
dc.contributor.authorFueyo, J.en
dc.contributor.authorGomez-Manzano, C.en
dc.contributor.authorVenkaiah, B.en
dc.contributor.authorBjerkvig, R.en
dc.contributor.authorYung, W. K.en
dc.contributor.authorSawaya, R.en
dc.contributor.authorKyritsis, A. P.en
dc.contributor.authorRao, J. S.en
dc.date.accessioned2015-11-24T19:37:35Z
dc.date.available2015-11-24T19:37:35Z
dc.identifier.issn0950-9232-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24051
dc.rightsDefault Licence-
dc.subjectAdenoviridae/geneticsen
dc.subjectAnimalsen
dc.subjectBrain Neoplasms/secondaryen
dc.subjectCollagenen
dc.subjectCyclin-Dependent Kinase Inhibitor p16/*genetics/physiologyen
dc.subjectDrug Combinationsen
dc.subjectGelatinases/*metabolismen
dc.subject*Gene Transfer Techniquesen
dc.subjectGenes, Tumor Suppressor/*geneticsen
dc.subjectGenetic Vectors/geneticsen
dc.subjectGlioma/*genetics/metabolism/*secondaryen
dc.subjectHumansen
dc.subjectLamininen
dc.subjectMatrix Metalloproteinase 2en
dc.subjectMetalloendopeptidases/*metabolismen
dc.subjectNeoplasm Invasivenessen
dc.subjectNeoplasm Proteins/*metabolismen
dc.subjectProteoglycansen
dc.subjectProto-Oncogene Proteins p21(ras)/metabolismen
dc.subjectRatsen
dc.subjectRats, Sprague-Dawleyen
dc.subjectRetinoblastoma Protein/metabolismen
dc.subjectSpheroids, Cellular/pathologyen
dc.subjectbeta-Galactosidase/genetics/metabolismen
dc.titleAdenovirus-mediated p16/CDKN2 gene transfer suppresses glioma invasion in vitroen
heal.abstractMalignant gliomas extensively infiltrate the surrounding normal brain, and their diffuse invasion is one of the most important barriers to successful therapy. Recent studies indicate that the progression of gliomas from low-grade to high-grade may depend on the acquisition of a new phenotype and the subsequent addition of genetic defects. One of the most frequent abnormalities in the progression of gliomas is the inactivation of tumor-suppressor gene p16, suggesting that loss of p16 is associated with acquisition of malignant characteristics. Consistent with this hypothesis, our previous studies showed that restoring wild-type p16 activity into p16-null malignant glioma cells modified their phenotype. In order to understand whether the biological consequences of p16 inactivation in high-grade gliomas included facilitating invasiveness, we used a recombinant replication-deficient adenovirus carrying the cDNA of the p16/CDKN2 gene to infect and express high levels of p16 protein in p16-null SNB19 glioma cells. Invasion of SNB19 glioma cells was tested into two models: invasion of glioma cells through Matrigel-coated transwell inserts and invasion of tumor-cell spheroids into fetal rat-brain aggregates in a co-culture system. Matrigel invasion assays showed that the SNB19 cells expressing exogenous p16 exhibited significantly reduced invasion. Similarly, invasion of p16-treated SNB19 cells into fetal rat-brain aggregates was reduced during a 72 h time period compared to invasion of the adenovirus-control and mock-infected cells. Expression of matrix metalloproteinase-2 (MMP-2), an enzyme involved in tumor-cell invasion, in SNB19 cells expressing p16 was significantly reduced compared to that of parental SNB19 and vector-infected cells. Our results show that restoring wild-type p16 activity into p16-null SNB19 glioma cells significantly inhibits tumor-cell invasion, thus suggesting a novel function of the p16 gene.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1038/sj.onc.1201382-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/9366522-
heal.identifier.secondaryhttp://www.nature.com/onc/journal/v15/n17/pdf/1201382a.pdf-
heal.journalNameOncogeneen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1997-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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