SV40 large T antigen up-regulates the retrotransposition frequency of viral-like 30 elements
| dc.contributor.author | Noutsopoulos, D. | en |
| dc.contributor.author | Vartholomatos, G. | en |
| dc.contributor.author | Kolaitis, N. | en |
| dc.contributor.author | Tzavaras, T. | en |
| dc.date.accessioned | 2015-11-24T19:43:33Z | |
| dc.date.available | 2015-11-24T19:43:33Z | |
| dc.identifier.issn | 0022-2836 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/24778 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Antigens, Polyomavirus Transforming/genetics/*physiology | en |
| dc.subject | Antigens, Viral, Tumor/genetics/physiology | en |
| dc.subject | Cell Line, Transformed | en |
| dc.subject | *Cell Transformation, Viral | en |
| dc.subject | Green Fluorescent Proteins/genetics/metabolism | en |
| dc.subject | Hygromycin B/metabolism | en |
| dc.subject | Mice | en |
| dc.subject | Moloney murine leukemia virus/enzymology | en |
| dc.subject | Mutation | en |
| dc.subject | NIH 3T3 Cells | en |
| dc.subject | Plasmids | en |
| dc.subject | RNA-Directed DNA Polymerase/genetics | en |
| dc.subject | Retroelements/genetics/*physiology | en |
| dc.subject | *Up-Regulation | en |
| dc.title | SV40 large T antigen up-regulates the retrotransposition frequency of viral-like 30 elements | en |
| heal.abstract | The regulation of non-autonomous retrotransposition is not known. A recombinant bearing a hygromycin gene and a viral-like 30 (VL30) retrotransposon tagged with an enhanced green fluorescent protein (EGFP) gene-based retrotransposition cassette was constructed and used for detection of retrotransposition events. Transfection of this recombinant produced retrotransposition events, detected both by EGFP fluorescence and PCR analysis, in hygromycin-selected clones of two established simian virus 40 (SV40)-transformed mouse NIH3T3 cell lines but not in normal NIH3T3 cells. The retrotransposition potential of this recombinant, as a provirus, was studied in stably transfected NIH3T3 clones. Transfection of these clones with either a wild-type or a mutant LE1135T SV40 large T antigen gene, not expressing small t protein, induced retrotransposition events at high frequencies as measured by fluorescence-activated cell scanning (FACS). In addition, measuring retrotransposition frequencies over a period of nine days following infection with isolated SV40 particles, revealed that the frequency of retrotransposition was time-dependent and induced as early as 24 h, increasing exponentially to high levels (>10(-2) events per cell per generation) up to nine days post-infection. Furthermore, ectopic expression of a cloned MoMLV-reverse transcriptase gene also produced retrotransposition events and suggested that the large T antigen most likely acted through induction of expression of endogenous reverse transcriptase genes. Our results show a direct correlation between SV40-cell transformation and VL30 retrotransposition and provide for the first time strong evidence that SV40 large T antigen up-regulates the retrotransposition of VL30 elements. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1016/j.jmb.2006.06.030 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/16859708 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0022283606007637/1-s2.0-S0022283606007637-main.pdf?_tid=4d9be4aed7abe3be0d0dcd8bfec61d1e&acdnat=1333007296_7d09388614ee81602d0f8636f005ab6b | - |
| heal.journalName | J Mol Biol | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2006 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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