p16INK4A is regularly expressed in Hodgkin's disease: comparison with retinoblastoma, p53 and MDM2 protein status, and the presence of Epstein-Barr virus

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dc.contributor.authorGuenova, M.en
dc.contributor.authorRassidakis, G. Z.en
dc.contributor.authorGorgoulis, V. G.en
dc.contributor.authorAngelopoulou, M. K.en
dc.contributor.authorSiakantaris, M. R.en
dc.contributor.authorKanavaros, P.en
dc.contributor.authorPangalis, G. A.en
dc.contributor.authorKittas, C.en
dc.date.accessioned2015-11-24T19:00:20Z
dc.date.available2015-11-24T19:00:20Z
dc.identifier.issn0893-3952-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/19513
dc.rightsDefault Licence-
dc.subjectAdolescenten
dc.subjectAdulten
dc.subjectAgeden
dc.subjectFemaleen
dc.subjectGene Expression Regulation, Neoplastic/*physiologyen
dc.subject*Genes, p16en
dc.subjectHerpesvirus 4, Human/genetics/*isolation & purificationen
dc.subjectHodgkin Disease/*genetics/virologyen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectIn Situ Hybridizationen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectNeoplasm Proteins/geneticsen
dc.subject*Nuclear Proteinsen
dc.subjectProto-Oncogene Proteins/geneticsen
dc.subjectProto-Oncogene Proteins c-mdm2en
dc.subjectRNA, Viral/geneticsen
dc.subjectRetinoblastoma Protein/geneticsen
dc.subjectTreatment Outcomeen
dc.subjectTumor Suppressor Protein p53/geneticsen
dc.titlep16INK4A is regularly expressed in Hodgkin's disease: comparison with retinoblastoma, p53 and MDM2 protein status, and the presence of Epstein-Barr virusen
heal.abstractIn order to understand better the possible role of cell-cycle regulating molecules in the pathogenesis of Hodgkin's disease (HD), the immunohistochemical distribution pattern of p16INK4A was investigated and compared with pRb, p53, and MDM2 protein status in 44 HD cases. Our findings were correlated to the presence of Epstein-Barr virus as detected by RNA in situ hybridization and clinicopathological parameters. p16INK4A protein immunoreactivity was found in all 44 cases with a proportion of Hodgkin-Reed-Sternberg (HRS) cells ranging from 30 to 90%. In 93% of the cases studied, pRb was detected in HRS, whereas all cases showed overexpression of p53. Almost all specimens (98%) were MDM2-positive as evaluated by 1B10 and/or IF2 monoclonal antibodies. EBER 1/2-transcripts were detected in 31.8% (14 of 44) of the examined samples. A significant correlation was observed between immunoreactivity of p16INK4A and MDM2 and the number of HRS cells (P = .0012 and P = .018, respectively). In a subgroup of cases, with p16INK4A expression in more than 50% of HRS cells, the percentage of pRb-positive neoplastic cells was inversely related to that of p16-positive ones (P = .007). No clinicopathological parameters or clinical prognostic indicators, including duration of response to therapy, were statistically related to the expression levels of any of the four proteins investigated or the presence of Epstein-Barr virus. Our findings suggest that p16 and pRb are regularly expressed and that their pathway in cell-cycle machinery seems to be intact in HD. However, further investigation is needed to shed light on the involvement of cell-cycle molecules in Hodgkin's lymphomagenesis and longer patient follow-up is required for conclusive prognostic correlation.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/10574604-
heal.journalNameMod Patholen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1999-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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