Glutamate receptor activation can trigger electrical activity in human glioma cells
Φόρτωση...
Ημερομηνία
Συγγραφείς
Labrakakis, C.
Patt, S.
Hartmann, J.
Kettenmann, H.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Eur J Neurosci
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Cells from major types of gliomas, i.e. oligodendrogliomas and glioblastomas, are able to generate action potentials upon a current injection similar to neurons (Patt et al. (1996) Neuroscience, 71, 601-611; Labrakakis et al. (1997b) J. Neuropath. Exp. Neurol., 56, 243-254. Here, we report that activation of ionotropic glutamate receptors by the selective agonist, kainate, or by glutamate itself, depolarized the tumour cells in culture and living slices from tumour tissue, and can elicit volleys of action potentials, as recorded with the patch-clamp technique. Sixty-six percent of the glioblastoma cells, 44% of the astocytoma and 86% of the oligodendroglioma cells responded to glutamate and the specific agonist of AMPA/kainate receptors, kainate. The involvement of non-NMDA (N-methyl-D-aspartate) receptors is further supported by the observation that both kainate and glutamate currents were blocked by CNQX (6-cyano-7-nitroquinoxaline-2,3-dione). The receptor activation was accompanied by an increase in cytosolic Ca2+, as recorded with a fura-2 microfluorometric system. The Ca2+ elevation was mediated by the activation of Ca2+ channels due to membrane depolarization. The presence of voltage-gated Ca2+ channels was confirmed by patch-clamp experiments. Taken together, these findings imply that the electrophysiological properties of glioma cells are more reminiscent of those of neurons than of glial cells.
Περιγραφή
Λέξεις-κλειδιά
Calcium/metabolism, Calcium Channels/metabolism, Electric Conductivity, Electrophysiology, Glioma/*metabolism/pathology/*physiopathology, Glutamic Acid/pharmacology, Humans, Intracellular Membranes/metabolism, Ion Channel Gating/physiology, Kainic Acid/
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/9753101
http://onlinelibrary.wiley.com/store/10.1046/j.1460-9568.1998.00226.x/asset/j.1460-9568.1998.00226.x.pdf?v=1&t=hh1pw9di&s=be072c276d606199ed9259aa5ff366a8df76fba4
http://onlinelibrary.wiley.com/store/10.1046/j.1460-9568.1998.00226.x/asset/j.1460-9568.1998.00226.x.pdf?v=1&t=hh1pw9di&s=be072c276d606199ed9259aa5ff366a8df76fba4
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών