Isotope dilution gas chromatographic-mass spectrometric method for the determination of lignans and isoflavonoids in human urine, including identification of genistein

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dc.contributor.authorAdlercreutz, H.en
dc.contributor.authorFotsis, T.en
dc.contributor.authorBannwart, C.en
dc.contributor.authorWahala, K.en
dc.contributor.authorBrunow, G.en
dc.contributor.authorHase, T.en
dc.date.accessioned2015-11-24T19:08:39Z
dc.date.available2015-11-24T19:08:39Z
dc.identifier.issn0009-8981-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/20561
dc.rightsDefault Licence-
dc.subject4-Butyrolactone/analogs & derivatives/urineen
dc.subjectButylene Glycols/urineen
dc.subjectChromans/urineen
dc.subjectEquolen
dc.subjectFemaleen
dc.subjectFurans/urineen
dc.subjectGas Chromatography-Mass Spectrometryen
dc.subjectGenisteinen
dc.subjectHumansen
dc.subjectIndicator Dilution Techniquesen
dc.subjectIsoflavones/chemistry/*urineen
dc.subjectLignansen
dc.subjectLignin/*analysisen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectMaleen
dc.titleIsotope dilution gas chromatographic-mass spectrometric method for the determination of lignans and isoflavonoids in human urine, including identification of genisteinen
heal.abstractWe describe an isotope dilution gas chromatographic-mass spectrometric method for the quantitative determination of the lignans enterolactone, enterodiol and matairesinol and the isoflavonoids daidzein, equol, O-desmethylangolensin and genistein in urine. Furthermore we present the gas chromatographic/mass spectrometer identification of genistein. Urine samples were extracted on Sep-Pak cartridges, conjugated fractions were isolated by chromatography on the acetate form of DEAE-Sephadex and deuterated internal standards of all seven compounds were added to the samples before hydrolysis. The hydrolysate was extracted on a Sep-Pak cartridge and following chromatography on the acetate form of QAE-Sephadex two fractions were obtained: Fraction 1 contained equol, enterolactone, enterodiol, matairesinol and all estrogens and fraction 2 contained O-desmethylangolensin, daidzein and genistein. The latter was ready for gas chromatography/mass spectrometry, but the first one was further purified to eliminate the estrogens by chromatography on the carbonate form of QAE-Sephadex. Following silylation, the samples were analyzed by combined capillary column gas chromatography/mass spectrometry in the selective ion monitoring mode. The within-assay imprecision varied from 0.8-15.2% (mean 8.7%) and the between-assay imprecision from 4.1-13.9% (mean 9.3%), depending on compound and concentration level. The mean recovery of authentic standards added to urine extracts before hydrolysis varied from 96.6 to 105.5%. Values obtained from 10 Finnish omnivorous men are presented. Individual values for matairesinol (excretion range 3.3-59.9 nmol/24 h) and genistein (range 21.8-1180 nmol/24 h) in human urine have never been published before.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/1663006-
heal.identifier.secondaryhttp://ac.els-cdn.com/0009898191901202/1-s2.0-0009898191901202-main.pdf?_tid=8f3d7d0fa25f81068ee4fea924b4e14c&acdnat=1332748310_95f2a9d88dd8113df2a8084146203453-
heal.journalNameClin Chim Actaen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate1991-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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