Dual function of rhoD in vesicular movement and cell motility

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dc.contributor.authorMurphy, C.en
dc.contributor.authorSaffrich, R.en
dc.contributor.authorOlivo-Marin, J. C.en
dc.contributor.authorGiner, A.en
dc.contributor.authorAnsorge, W.en
dc.contributor.authorFotsis, T.en
dc.contributor.authorZerial, M.en
dc.date.accessioned2015-11-24T19:23:32Z
dc.date.available2015-11-24T19:23:32Z
dc.identifier.issn0171-9335-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22326
dc.rightsDefault Licence-
dc.subjectActin Cytoskeleton/*metabolismen
dc.subjectAnimalsen
dc.subjectCell Compartmentation/*physiologyen
dc.subjectCell Movement/*physiologyen
dc.subjectCells, Cultured/cytology/metabolismen
dc.subjectCricetinaeen
dc.subjectEndosomes/*metabolism/ultrastructureen
dc.subjectEndothelium/cytology/metabolismen
dc.subjectGene Expression/physiologyen
dc.subjectGenetic Vectorsen
dc.subjectGreen Fluorescent Proteinsen
dc.subjectIndicators and Reagents/pharmacokineticsen
dc.subjectIntracellular Membranes/*metabolism/ultrastructureen
dc.subjectLuminescent Proteins/pharmacokineticsen
dc.subjectLysosomes/metabolismen
dc.subjectMicroscopy, Videoen
dc.subjectProtein Transport/*physiologyen
dc.subjectProteins/genetics/*metabolismen
dc.subjectReceptors, Transferrin/genetics/metabolismen
dc.subjectTransfectionen
dc.subject*rho GTP-Binding Proteinsen
dc.titleDual function of rhoD in vesicular movement and cell motilityen
heal.abstractThe trafficking of intracellular membranes requires the coordination of membrane-cytoskeletal interactions. Rab proteins are key players in the regulation of vesicular transport, while Rho family members control actin-dependent cell functions. We have previously identified a rho protein, rhoD, which is localized to the plasma membrane and early endosomes. When overexpressed, rhoD alters the actin cytoskeleton and plays an important role in endosome organization. We found that a rhoD mutant exerts its effect on early endosome dynamics through an inhibition in organelle motility. In these studies, the effect of rhoD on endosome dynamics was evaluated in the presence of a constitutively active, GTPase-deficient mutant of rab5, rab5Q79L. As rab5Q79L itself stimulates endosome motility, rhoD might counteract this stimulation, without itself exerting any effect in the absence of rab5 activation. We have now addressed this issue by investigating the effect of rhoD in the absence of co-expressed rab5. We find that rhoDG26V alone alters vesicular dynamics. Vesicular movement, in particular the endocytic/recycling circuit, is altered during processes such as cell motility. Due to the participation of vesicular motility and cytoskeletal rearrangements in cell movement and the involvement of rhoD in both, we have addressed the role of rhoD in this process and have found that rhoDG26V inhibits endothelial cell motility.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11484930-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0171933504701557/1-s2.0-S0171933504701557-main.pdf?_tid=92cf0352efa6a59aaf2921cce0d86b87&acdnat=1332753172_fd88747d0e8de327d4b34c3942ade3af-
heal.journalNameEur J Cell Biolen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2001-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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